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  Preparation of Biological Samples for Electron Microscopy

Schwarz, H., & Humbel, B. (2008). Preparation of Biological Samples for Electron Microscopy. In M. Luysberg, K. Tillmann, & T. Weirich (Eds.), EMC 2008 14th European Microscopy Congress 1–5 September 2008 (pp. 799-800). Berlin, Germany: Springer.

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Datensatz-Permalink: https://hdl.handle.net/21.11116/0000-000D-0BFD-B Versions-Permalink: https://hdl.handle.net/21.11116/0000-000D-0BFE-A
Genre: Konferenzbeitrag

Externe Referenzen

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Urheber

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 Urheber:
Schwarz, H1, Autor           
Humbel, BM, Autor
Affiliations:
1Electron Microscopy, Max Planck Institute for Developmental Biology, Max Planck Society, ou_3375794              

Inhalt

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Schlagwörter: -
 Zusammenfassung: Biological structures of living organisms are highly dynamic, they are always in an aqueous environment and the components are mainly made of elements of low atomic number: H, C, O, N, P, S. Last but not least most of the organism are rather large. The physical properties of electrons define the preparation methods of biological samples. Electrons have a short mean free pathway of about 1 cm in air and even shorter in denser structures, therefore the electron microscope needs to be evacuated. Thus an ideal sample for electron microscopy is tiny, does not move, is rigid, contains no liquids and has a high contrast. In addition for transmission electron microscopy the sample must be thin. The opposing properties and demands of electrons versus biology led to cumbersome path on biological sample preparation. In the beginning viruses and bacteria were studied [1, 2]. They were adsorbed on support film and initially directly imaged with the electron beam. The poor contrast was improved by heavy metal shadowing. For the visualization of molecules and virus particles in the 50ies the spreading technique introduced by Kleinschmidt [3] as well as the negative staining technique pioneered by Brenner & Horne [4] were cornerstones.

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Sprache(n):
 Datum: 2008
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: DOI: 10.1007/978-3-540-85156-1_400
 Art des Abschluß: -

Veranstaltung

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Titel: 14th European Microscopy Congress (EMC 2008)
Veranstaltungsort: Aachen, Germany
Start-/Enddatum: 2008-09-01 - 2008-09-05

Entscheidung

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Projektinformation

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Quelle 1

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Titel: EMC 2008 14th European Microscopy Congress 1–5 September 2008
Genre der Quelle: Konferenzband
 Urheber:
Luysberg, M, Herausgeber
Tillmann, K, Herausgeber
Weirich, T, Herausgeber
Affiliations:
-
Ort, Verlag, Ausgabe: Berlin, Germany : Springer
Seiten: - Band / Heft: - Artikelnummer: - Start- / Endseite: 799 - 800 Identifikator: ISBN: 978-3-540-85154-7