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  Using Bioinformatics and Molecular Biology to Streamline Construction of Effector Libraries for Phytopathogenic Pseudomonas syringae Strains

Jayaraman, J., Halane, M., Choi, S., McCann, H., & Sohn, K. (1991). Using Bioinformatics and Molecular Biology to Streamline Construction of Effector Libraries for Phytopathogenic Pseudomonas syringae Strains. In W. Gassmann (Ed.), Plant Innate Immunity: Methods and Protocols (pp. 1-12). New York, NY, USA: Humana Press.

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 Creators:
Jayaraman, J, Author
Halane, MK, Author
Choi, S, Author
McCann, HC1, Author                 
Sohn, KH, Author
Affiliations:
1External Organizations, ou_persistent22              

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 Abstract: The war between plants and their pathogens is endless, with plant resistance genes offering protection against pathogens until the pathogen evolves a way to overcome this resistance. Given how quickly new pathogen strains can arise and defeat plant defenses, it is critical to more rapidly identify and examine the specific genomic characteristics new virulent strains have gained which give them the upper hand. An indispensable tool is bioinformatics. Genome sequencing has advanced rapidly in the last decade, and labs are frequently uploading high-quality genomes of various organisms, including plant pathogenic bacteria such as Pseudomonas syringae. Pseudomonas syringae strains inject several effector proteins into host cells which often overcome host defenses. Probing online genomes provides a way to quickly and accurately predict effector repertoires of Pseudomonas, enabling the cloning of complete effector libraries of newly emerged strains. Here, we describe detailed protocols to rapidly clone bioinformatically predicted P. syringae effectors for various screening applications.

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 Dates: 1991
 Publication Status: Issued
 Pages: -
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 Rev. Type: -
 Identifiers: DOI: 10.1007/978-1-4939-9458-8_1
PMID: 31041757
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Title: Plant Innate Immunity: Methods and Protocols
Source Genre: Book
 Creator(s):
Gassmann, W, Editor
Affiliations:
-
Publ. Info: New York, NY, USA : Humana Press
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 1 - 12 Identifier: ISBN: 978-1-4939-9457-1
DOI: 10.1007/978-1-4939-9458-8

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Title: Methods in Molecular Biology
  Other : Methods Mol. Biol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: US : Springer
Pages: - Volume / Issue: 1991 Sequence Number: - Start / End Page: - Identifier: ISSN: 1064-3745
CoNE: https://pure.mpg.de/cone/journals/resource/954927725544