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  Biosynthesis of the multifunctional isopropylstilbene in Photorhabdus laumondii involves cross-talk between specialized and primary metabolism

Kavakli, S., Grammbitter, G. L. C., & Bode, H. B. (2022). Biosynthesis of the multifunctional isopropylstilbene in Photorhabdus laumondii involves cross-talk between specialized and primary metabolism. bioRxiv: the preprint server for biology, 2022.10.20.513026.

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Kavakli, Siyar1, Author
Grammbitter, Gina L. C.1, Author
Bode, Helge B.2, 3, 4, 5, Author                 
Affiliations:
1external, ou_persistent22              
2Chemical Biology, Department of Chemistry, Philipps University Marburg, Marburg, Germany, ou_persistent22              
3Molecular Biotechnology, Department of Biosciences, Goethe University Frankfurt, Frankfurt, Germany, External Organizations, ou_421891              
4Senckenberg Gesellschaft für Naturforschung, Frankfurt, ou_persistent22              
5Natural Product Function and Engineering, Department of Natural Products in Organismic Interactions, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266308              

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 Abstract: Isopropylstilbene (IPS) derived from the entomopathogenic bacterium Photorhabdus represents the only known stilbene which is not produced by a plant stilbene synthase but a bacterial PKS II synthase. While the exclusive cyclization reaction, responsible for the formation of the characteristic iso-branched side-chain of the molecule, was studied in the past, some parts of the biosynthetic route remained elusive. In this study, we revealed the role of StlB that is able to produce CoA-derivatives and demonstrated the elongation of cinnamoyl-CoA with enzymes from the bacterial fatty acid biosynthesis pathway. Thus, we deciphered cross-talk between the enzymes from primary and specialized metabolism. These insights led, for the first time, to the production of IPS in a heterologous host.Competing Interest StatementThe authors have declared no competing interest.

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Language(s): eng - English
 Dates: 2022-10-21
 Publication Status: Issued
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 Rev. Type: No review
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Title: bioRxiv : the preprint server for biology
  Abbreviation : bioRxiv
Source Genre: Journal
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Pages: - Volume / Issue: - Sequence Number: 2022.10.20.513026 Start / End Page: - Identifier: ZDB: 2766415-6
CoNE: https://pure.mpg.de/cone/journals/resource/2766415-6