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  Sample Preparation for Time-Resolved Serial Crystallography: Practical Considerations

Mehrabi, P., & Schulz, E.-C. (2023). Sample Preparation for Time-Resolved Serial Crystallography: Practical Considerations. In A. Sousa, & L. Passarinha (Eds.), Advanced Methods in Structural Biology (pp. 361-379). Berlin/Heidelberg: Springer Science+Business Media. doi:10.1007/978-1-0716-3147-8_21.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000D-10F5-C Version Permalink: https://hdl.handle.net/21.11116/0000-000D-10F6-B
Genre: Book Chapter

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978-1-0716-3147-8_21.pdf (Publisher version), 216KB
 
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https://doi.org/10.1007/978-1-0716-3147-8_21 (Publisher version)
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 Creators:
Mehrabi, P.1, 2, Author           
Schulz, E.-C.2, 3, Author           
Affiliations:
1Institute for Nanostructure and Solid State Physics, Universität Hamburg, ou_persistent22              
2Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_1938288              
3University Medical Center Hamburg-Eppendorf (UKE), ou_persistent22              

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Free keywords: Time-resolved crystallography; Serial crystallography; SSX; Structural biology; Structural enzymology
 Abstract: Time-resolved serial crystallography is an emerging method to elucidate the structure-function relationship of biomolecular systems at up to atomic resolution. However, to make this demanding method a success, a number of experimental requirements have to be met. In this chapter, we summarize general guidelines and protocols towards performing time-resolved crystallography experiments, with a particular emphasis on sample requirements and preparation but also a brief excursion into reaction initiation.

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Language(s): eng - English
 Dates: 2023-04-252023-04-25
 Publication Status: Issued
 Pages: 19
 Publishing info: -
 Table of Contents: -
 Rev. Type: Internal
 Identifiers: DOI: 10.1007/978-1-0716-3147-8_21
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Project name : The authors gratefully acknowledge the support provided by the Max Planck Society. PM acknowledges support from the Deutsche Forschungsgemeinschaft (DFG) via grant No. 451079909 and from a Joachim Herz Stiftung add-on fellowship. ES acknowledges support by the DFG via grant No. 458246365, and by the Federal Ministry of Education and Research, Germany, under grant number 01KI2114.
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Title: Advanced Methods in Structural Biology
Source Genre: Book
 Creator(s):
Sousa, A., Editor
Passarinha, L., Editor
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Publ. Info: Berlin/Heidelberg : Springer Science+Business Media
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 361 - 379 Identifier: ISBN: 978-1-0716-3146-1