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  Crystal structure of FAD-independent methylene-tetrahydrofolate reductase from Mycobacterium hassiacum

Gehl, M., Demmer, U., Ermler, U., & Shima, S. (2023). Crystal structure of FAD-independent methylene-tetrahydrofolate reductase from Mycobacterium hassiacum. Proteins: Structure, Function, and Bioinformatics. doi:10.1002/prot.26504.

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 Creators:
Gehl, Manuel1, Author                 
Demmer, Ulrike2, Author                 
Ermler, Ulrich2, Author                 
Shima, Seigo1, Author                 
Affiliations:
1Department-Independent Research Group Microbial Protein Structure, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266277              
2Max Planck Institute of Biophysics, Max Planck Society, Max-von-Laue-Straße 3, 60438 Frankfurt am Main, DE, ou_2068288              

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Free keywords: tuberculosis, methylene-tetrahydrofolate reductase, crystal structure, drug design, divergent evolution
 Abstract: FAD-independent methylene-tetrahydrofolate (methylene-H4F) reductase (Mfr), recently identified in mycobacteria, catalyzes the reduction of methylene-H4F to methyl-H4F with NADH as hydride donor by a ternary complex mechanism. This biochemical reaction corresponds to that of the ubiquitous FAD-dependent methylene-H4F reductase (MTHFR), although the latter uses a ping-pong mechanism with FAD as prosthetic group as intermediate hydride carrier. Comparative genomics and genetic analyses indicated that Mfr is indispensable for the growth of Mycobacterium tuberculosis, which lacks the MTHFR-encoding gene. Therefore, Mfr appears to be an excellent target for the design of antimycobacterial drugs. Here, we report the heterologous production, enzymological characterization, and the crystal structure of Mfr from the thermophilic mycobacterium Mycobacterium hassiacum (hMfr), which shows 78% sequence identity to Mfr from M. tuberculosis. Although hMfr and MTHFR have minor sequence identity and different catalytic mechanisms, their structures are highly similar, thus suggesting a divergent evolution of Mfr and MTHFR from a common ancestor. Most of the important active site residues of MTHFR are conserved and equivalently positioned in the tertiary structure of hMfr. The Glu9Gln variant of hMfr exhibits a drastic reduction of the catalytic activity, which supports the predicted function of the glutamate residue as proton donor in both hMfr and MTHFR. Thus, highly similar binding modes for the C1-carriers and the reducing agents in hMfr and MTHFR are assumed.

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Language(s): eng - English
 Dates: 2023-01-102023-04-132023-04-29
 Publication Status: Published online
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1002/prot.26504
PMID: 37119125
 Degree: -

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Title: Proteins: Structure, Function, and Bioinformatics
Source Genre: Journal
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Publ. Info: New York, NY : John Wiley & Sons
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: ISSN: 0887-3585
CoNE: https://pure.mpg.de/cone/journals/resource/954925553393_1