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  CBP and Gcn5 drive zygotic genome activation independently of their catalytic activity

Ciabrelli, F., Rabbani, L., Cardamone, F., Zenk, F., Löser, E., Schächtle, M. A., et al. (2023). CBP and Gcn5 drive zygotic genome activation independently of their catalytic activity. Science Advances, 9: eadf2687. doi:10.1126/sciadv.adf2687.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000D-12AB-E Version Permalink: https://hdl.handle.net/21.11116/0000-000D-12AE-B
Genre: Journal Article

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2023
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The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works
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https://creativecommons.org/licenses/by-nc/4.0/

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 Creators:
Ciabrelli, Filippo1, Author
Rabbani, Leily1, Author
Cardamone, Francesco1, Author
Zenk, Fides1, Author
Löser, Eva1, Author
Schächtle, Melanie A1, Author
Mazina, Marina1, Author
Loubiere, Vincent2, Author
Iovino, Nicola1, Author           
Affiliations:
1Department of Chromatin Regulation, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243643              
2External Organizations, ou_persistent22              

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 Abstract: Zygotic genome activation (ZGA) is a crucial step of embryonic development. So far, little is known about the role of chromatin factors during this process. Here, we used an in vivo RNA interference reverse genetic screen to identify chromatin factors necessary for embryonic development in Drosophila melanogaster. Our screen reveals that histone acetyltransferases (HATs) and histone deacetylases are crucial ZGA regulators. We demonstrate that Nejire (CBP/EP300 ortholog) is essential for the acetylation of histone H3 lysine-18 and lysine-27, whereas Gcn5 (GCN5/PCAF ortholog) for lysine-9 of H3 at ZGA, with these marks being enriched at all actively transcribed genes. Nonetheless, these HATs activate distinct sets of genes. Unexpectedly, individual catalytic dead mutants of either Nejire or Gcn5 can activate zygotic transcription (ZGA) and transactivate a reporter gene in vitro. Together, our data identify Nejire and Gcn5 as key regulators of ZGA.

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Language(s): eng - English
 Dates: 2023-04-21
 Publication Status: Published online
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1126/sciadv.adf2687
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Title: Science Advances
  Other : Sci. Adv.
Source Genre: Journal
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Publ. Info: Washington : AAAS
Pages: - Volume / Issue: 9 Sequence Number: eadf2687 Start / End Page: - Identifier: ISSN: 2375-2548
CoNE: https://pure.mpg.de/cone/journals/resource/2375-2548