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  A practical guideline to engineering nonribosomal peptide synthetases

Abbood, N., Präve, L., Bozhüyük, K. A. J., & Bode, H. B. (2023). A practical guideline to engineering nonribosomal peptide synthetases. In M. Burkart, & F. Ishikawa (Eds.), Non-Ribosomal Peptide Biosynthesis and Engineering. Methods in Molecular Biology,. New York: Humana, New York, NY.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000D-2E97-6 Version Permalink: https://hdl.handle.net/21.11116/0000-000E-40B3-F
Genre: Book Chapter

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https://doi.org/10.1007/978-1-0716-3214-7_11 (Publisher version)
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 Creators:
Abbood, Nadya1, Author           
Präve, Leonard1, Author           
Bozhüyük, Kenan A. J.1, Author           
Bode, Helge B.1, 2, 3, 4, Author                 
Affiliations:
1Natural Product Function and Engineering, Department of Natural Products in Organismic Interactions, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266308              
2Chemical Biology, Department of Chemistry, Philipps University Marburg, Marburg, Germany, ou_persistent22              
3Senckenberg Gesellschaft für Naturforschung, Frankfurt, ou_persistent22              
4Molecular Biotechnology, Department of Biosciences, Goethe University Frankfurt, Frankfurt, Germany, External Organizations, ou_421891              

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 Abstract: The bioengineering of nonribosomal peptide synthetases (NRPSs) is a rapidly developing field to access natural product derivatives and new-to-nature natural products like scaffolds with changed or improved properties. However, the rational (re-)design of these often gigantic assembly-line proteins is by no means trivial and needs in-depth insights into structural flexibility, inter-domain communication, and the role of proofreading by catalytic domains-so it is not surprising that most previous rational reprogramming efforts have been met with limited success. With this practical guide, the result of nearly one decade of NRPS engineering in the Bode lab, we provide valuable insights into the strategies we have developed during this time for the successful engineering and cloning of these fascinating molecular machines. © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

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Language(s): eng - English
 Dates: 2023-05-16
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 37184707
DOI: 10.1007/978-1-0716-3214-7_11
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Title: Non-Ribosomal Peptide Biosynthesis and Engineering. Methods in Molecular Biology,
Source Genre: Book
 Creator(s):
Burkart, M., Editor
Ishikawa, F., Editor
Affiliations:
-
Publ. Info: New York : Humana, New York, NY
Pages: - Volume / Issue: 2670 Sequence Number: - Start / End Page: - Identifier: ISSN: 1940-6029