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  Structural changes in chromosomes driven by multiple condensin motors during mitosis

Dey, A., Shi, G., Takaki, R., & Thirumalai, D. (2023). Structural changes in chromosomes driven by multiple condensin motors during mitosis. Cell Reports, 42(4): 112348. doi:10.1016/j.celrep.2023.112348.

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 Creators:
Dey, Atreya1, Author
Shi, Guang1, Author
Takaki, Ryota2, Author           
Thirumalai, D.1, Author
Affiliations:
1external, ou_persistent22              
2Max Planck Institute for the Physics of Complex Systems, Max Planck Society, ou_2117288              

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 Abstract: We create a computational framework that utilizes loop extrusion (LE) by multiple condensin I/II motors to predict changes in chromosome organization during mitosis. The theory accurately reproduces the experi-mental contact probability profiles for the mitotic chromosomes in HeLa and DT40 cells. The LE rate is smaller at the start of mitosis and increases as the cells approach metaphase. Condensin II-mediated mean loop size is about six times larger than loops because of condensin I. The loops, which overlap each other, are stapled to a central dynamically changing helical scaffold formed by the motors during the LE process. A polymer physics-based data-driven method that uses the Hi-C contact map as the only input shows that the helix is characterized as random helix perversions (RHPs) in which the handedness changes randomly along the scaffold. The theoretical predictions, which are testable using imaging experiments, do not contain any parameters.

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Language(s): eng - English
 Dates: 2023-04-062023-04-06
 Publication Status: Issued
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Title: Cell Reports
Source Genre: Journal
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Publ. Info: Maryland Heights, MO : Cell Press
Pages: - Volume / Issue: 42 (4) Sequence Number: 112348 Start / End Page: - Identifier: ISSN: 2211-1247
CoNE: https://pure.mpg.de/cone/journals/resource/2211-1247