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  High-contrast en bloc staining of mouse whole-brain and human brain samples for EM-based connectomics

Song, K., Feng, Z., & Helmstaedter, M. (2023). High-contrast en bloc staining of mouse whole-brain and human brain samples for EM-based connectomics. Nat. Methods. doi:10.1038/s41592-023-01866-3.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000D-3E68-A Version Permalink: https://hdl.handle.net/21.11116/0000-000D-3E6A-8
Genre: Journal Article

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https://pubmed.ncbi.nlm.nih.gov/37156843/ (Any fulltext)
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 Creators:
Song, Kun1, Author
Feng, Zhihui1, Author
Helmstaedter, Moritz1, Author           
Affiliations:
1Connectomics Department, Max Planck Institute for Brain Research, Max Planck Society, ou_2461695              

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 Abstract: Connectomes of human cortical gray matter require high-contrast homogeneously stained samples sized at least 2 mm on a side, and a mouse whole-brain connectome requires samples sized at least 5-10 mm on a side. Here we report en bloc staining and embedding protocols for these and other applications, removing a key obstacle for connectomic analyses at the mammalian whole-brain level.

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Language(s): eng - English
 Dates: 2022-02-282023-03-272023-05-08
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s41592-023-01866-3
PMID: 37156843
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Title: Nat. Methods
  Other : Nature Methods
Source Genre: Journal
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Publ. Info: New York, NY : Nature Publishing Group
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: ISSN: 1548-7091
CoNE: https://pure.mpg.de/cone/journals/resource/111088195279556