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  Chromosome Conformation Capture Followed by Genome-Wide Sequencing (Hi-C) in Drosophila Embryos

Cardamone, F., Zhan, Y., Iovino, N., & Zenk, F. (2023). Chromosome Conformation Capture Followed by Genome-Wide Sequencing (Hi-C) in Drosophila Embryos. Methods in Molecular Biology, 2655, 41-55. doi:10.1007/978-1-0716-3143-0_4.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000D-4548-5 Version Permalink: https://hdl.handle.net/21.11116/0000-000D-4549-4
Genre: Journal Article

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10.1007_978-1-0716-3143-0_4.pdf (Publisher version), 10MB
 
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10.1007_978-1-0716-3143-0_4.pdf
Description:
pages 41-55
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Restricted (Max Planck Institute of Immunobiology and Epigenetics, MFIB; )
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application/pdf
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Copyright Date:
2023
Copyright Info:
The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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https://link.springer.com/protocol/10.1007/978-1-0716-3143-0_4 (Supplementary material)
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 Creators:
Cardamone, Francesco1, Author
Zhan, Yinxiu2, Author
Iovino, Nicola1, Author           
Zenk, Fides2, Author
Affiliations:
1Department of Chromatin Regulation, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243643              
2External Organizations, ou_persistent22              

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Free keywords: Chromosome conformation capture; Drosophila embryos; Hi-C
 Abstract: This protocol provides specific details on how to perform Hi-C, the genome-wide version of Chromosome Conformation Capture (3C) followed by high-throughput sequencing, in Drosophila embryos. Hi-C provides a genome-wide population-averaged snapshot of the 3D genome organization within nuclei. In Hi-C, formaldehyde-cross-linked chromatin is enzymatically digested using restriction enzymes; digested fragments are biotinylated and subjected to proximity ligation; ligated fragments are purified using streptavidin followed by paired-end sequencing. Hi-C allows the detection of higher order folding structures such as topologically associated domains (TADs) and active/inactive compartments (A/B compartments, respectively). Performing this assay in developing embryos gives the unique opportunity to investigate dynamic chromatin changes when 3D chromatin structure is established in embryogenesis.

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Language(s): eng - English
 Dates: 2023-05-23
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1007/978-1-0716-3143-0_4
 Degree: -

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Title: Methods in Molecular Biology
  Other : Methods Mol. Biol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: US : Springer
Pages: - Volume / Issue: 2655 Sequence Number: - Start / End Page: 41 - 55 Identifier: ISSN: 1064-3745
CoNE: https://pure.mpg.de/cone/journals/resource/954927725544