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  Phylogroup-specific variation shapes the clustering of antimicrobial resistance genes and defence systems across regions of genome plasticity in Pseudomonas aeruginosa

Botelho, J., Tüffers, L., Fuss, J., Buchholz, F., Utpatel, C., Klockgether, J., Niemann, S., Tümmler, B., & Schulenburg, H. (2023). Phylogroup-specific variation shapes the clustering of antimicrobial resistance genes and defence systems across regions of genome plasticity in Pseudomonas aeruginosa. EBioMedicine, 90:. doi:10.1016/j.ebiom.2023.104532.

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アイテムのパーマリンク: https://hdl.handle.net/21.11116/0000-000D-4D40-5 版のパーマリンク: https://hdl.handle.net/21.11116/0000-000D-4D41-4
資料種別: 学術論文

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 作成者:
Botelho, João1, 著者           
Tüffers, Leif, 著者
Fuss, Janina, 著者
Buchholz, Florian, 著者
Utpatel, Christian, 著者
Klockgether, Jens, 著者
Niemann, Stefan, 著者
Tümmler, Burkhard, 著者
Schulenburg, Hinrich1, 著者                 
所属:
1Max Planck Fellow Group Antibiotic Resistance Evolution, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_2600692              

内容説明

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キーワード: Pangenome; Pseudomonas aeruginosa; Regions of genome plasticity; Antibiotic resistance; CRISPR-Cas systems; Defence systems
 要旨: Background

Pseudomonas aeruginosa is an opportunistic pathogen consisting of three phylogroups (hereafter named
A, B, and C). Here, we assessed phylogroup-specific evolutionary dynamics across available and also new
P. aeruginosa genomes.

Methods

In this genomic analysis, we first generated new genome assemblies for 18 strains of the major P. aeruginosa
clone type (mPact) panel, comprising a phylogenetically diverse collection of clinical and environmental isolates for
this species. Thereafter, we combined these new genomes with 1991 publicly available P. aeruginosa genomes for a
phylogenomic and comparative analysis. We specifically explored to what extent antimicrobial resistance (AMR)
genes, defence systems, and virulence genes vary in their distribution across regions of genome plasticity (RGPs) and
“masked” (RGP-free) genomes, and to what extent this variation differs among the phylogroups.

Findings

We found that members of phylogroup B possess larger genomes, contribute a comparatively larger number
of pangenome families, and show lower abundance of CRISPR-Cas systems. Furthermore, AMR and defence
systems are pervasive in RGPs and integrative and conjugative/mobilizable elements (ICEs/IMEs) from
phylogroups A and B, and the abundance of these cargo genes is often significantly correlated. Moreover, interand intra-phylogroup interactions occur at the accessory genome level, suggesting frequent recombination events.
Finally, we provide here the mPact panel of diverse P. aeruginosa strains that may serve as a valuable reference for
functional analyses.

Interpretation

Altogether, our results highlight distinct pangenome characteristics of the P. aeruginosa phylogroups,
which are possibly influenced by variation in the abundance of CRISPR-Cas systems and are shaped by the
differential distribution of other defence systems and AMR genes.

資料詳細

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言語: eng - English
 日付: 2023-03-022022-06-302023-03-072023-03-212023-04
 出版の状態: 出版
 ページ: -
 出版情報: -
 目次: -
 査読: -
 識別子(DOI, ISBNなど): DOI: 10.1016/j.ebiom.2023.104532
 学位: -

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出版物 1

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出版物名: EBioMedicine
種別: 学術雑誌
 著者・編者:
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出版社, 出版地: Amsterdam : Elsevier
ページ: - 巻号: 90 通巻号: 104532 開始・終了ページ: - 識別子(ISBN, ISSN, DOIなど): ISSN: 2352-3964
CoNE: https://pure.mpg.de/cone/journals/resource/2352-3964