English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Rapid and sequential movement of individual chromosomal loci to specific subcellular locations during bacterial DNA replication

Viollier, P., Thanbichler, M., McGrath, P., West, L., Meewan, M., McAdams, H., et al. (2004). Rapid and sequential movement of individual chromosomal loci to specific subcellular locations during bacterial DNA replication. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 101(25), 9257-9262. doi:10.1073/pnas.0402606101.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Viollier, PH1, Author
Thanbichler, M2, Author                 
McGrath, PT1, Author
West, L1, Author
Meewan, M1, Author
McAdams, HH1, Author
Shapiro, L1, Author
Affiliations:
1external, ou_persistent22              
2Department of Developmental Biology, Stanford University School of Medicine, Stanford, USA, ou_persistent22              

Content

show
hide
Free keywords: -
 Abstract: The chromosomal origin and terminus of replication are precisely
localized in bacterial cells. We examined the cellular position of 112
individual loci that are dispersed over the circular Caulobacter
crescentus chromosome and found that in living cells each locus has a
specific subcellular address and that these loci are arrayed in linear
order along the long axis of the cell. Time-lapse microscopy of the
location of the chromosomal origin and 10 selected loci in the
origin-proximal half of the chromosome showed that during DNA
replication, as the replisome sequentially copies each locus, the newly
replicated DNA segments are moved in chronological order to their final
subcellular destination in the nascent half of the predivisional cell.
Thus, the remarkable organization of the chromosome is being established
while DNA replication is still in progress. The fact that the movement
of these 10 loci is, like that of the origin, directed and rapid, and
occurs at a similar rate, suggests that the same molecular machinery
serves to partition and place many, if not most, chromosomal loci at
defined subcellular sites.

Details

show
hide
Language(s):
 Dates: 2004-06-22
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000222278600018
DOI: 10.1073/pnas.0402606101
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 101 (25) Sequence Number: - Start / End Page: 9257 - 9262 Identifier: ISSN: 0027-8424