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Abstract:
A polyphasic approach was used in which genotypic and phenotypic
properties of a gram-negative, obligately anaerobic, rod-shaped
bacterium isolated from a black anoxic freshwater mud sample were
determined. Based on these results, the name Holophaga foetida gen.
nov., sp. nov. is proposed. This microorganism produced dimethylsulfide
and methanethiol during growth on trimethoxybenzoate or syringate. The
only other compounds utilized were pyruvate and trihydroxybenzenes such
as gallate, phloroglucinol, or pyrogallol. The aromatic compounds were
degraded to acetate. Although comparison of the signature nucleotide
pattern of the five established subclasses of Proteobacteria with the
16S rDNA sequence of Holophaga foetida revealed a relationship to
members of the delta-subclass, the phylogenetic position within the
radiation of this class is so deep and dependent upon the number and
selection of reference sequences that its affiliation to the
Proteobacteria must be considered tentative. The type strain is H.
foetida strain TMBS4 (DSM 6591).
