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Abstract:
The complete nucleotide sequences of the Mycobacterium leprae 23 S and 5
S rRNA genes and their flanking regions are presented. As compared to
other eubacterial homologous molecules the 23 S rDNA exhibits two
insertions. A 16 nucleotide long insertion is almost unique to members
of the genus Mycobacterium, while the second represents an extended
version of helix 54. The potential of both insertions to serve as
target for diagnostic oligonucleotide probes was proven by comparative
sequence analysis of 23 S rRNA of several Mycobacterium species and by
dot blot hybridization. In addition, a 19-mer oligonucleotide probe is
described, which can be considered genus Mycobacterium-specific.
