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  A Rapid LC-MS/MS-PRM Assay for Serologic Quantification of Sialylated O-HPX Glycoforms in Patients with Liver Fibrosis

Panigrahi, A., Benicky, J., Wei, R., Ahn, J., Goldman, R., & Sanda, M. (2022). A Rapid LC-MS/MS-PRM Assay for Serologic Quantification of Sialylated O-HPX Glycoforms in Patients with Liver Fibrosis. MOLECULES, 27(7): 2213. doi:10.3390/molecules27072213.

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 Creators:
Panigrahi, Aswini, Author
Benicky, Julius, Author
Wei, Renhuizi, Author
Ahn, Jaeil, Author
Goldman, Radoslav, Author
Sanda, Miloslav1, Author           
Affiliations:
1Biomolecular Mass Spectrometry, Max Planck Institute for Heart and Lung Research, Max Planck Society, ou_2591705              

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 Abstract: Development of high throughput robust methods is a prerequisite for a successful clinical use of LC-MS/MS assays. In earlier studies, we reported that nLC-MS/MS measurement of the O-glycoforms of HPX is an indicator of liver fibrosis. In this study, we show that a microflow LC-MS/MS method using a single column setup for capture of the analytes, desalting, fast gradient elution, and on-line mass spectrometry measurements, is robust, substantially faster, and even more sensitive than our nLC setup. We demonstrate applicability of the workflow on the quantification of the O-HPX glycoforms in unfractionated serum samples of control and liver disease patients. The assay requires microliter volumes of serum samples, and the platform is amenable to one hundred sample injections per day, providing a valuable tool for biomarker validation and screening studies.

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 Dates: 2022-03-29
 Publication Status: Published online
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Title: MOLECULES
Source Genre: Journal
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Pages: - Volume / Issue: 27 (7) Sequence Number: 2213 Start / End Page: - Identifier: -