Computer-aided discovery of a novel thermophilic laccase for low-density 
polyethylene degradation

Zhang, Yan; Plesner, Thea Jess; Ouyang, Yi; Zheng, Yu-Cong; Bouhier, Etienne; Berentzen, Emilie Ingemann; Zhang, Mingliang; Zhou, Pengfei; Zimmermann, Wolfgang; Andersen, Gregers Rom; Eser, Bekir Engin; Guo, Zheng

doi:10.1016/j.jhazmat.2023.131986

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Computer-aided discovery of a novel thermophilic laccase for low-density polyethylene degradation

Zhang, Y., Plesner, T. J., Ouyang, Y., Zheng, Y.-C., Bouhier, E., Berentzen, E. I., et al. (2023). Computer-aided discovery of a novel thermophilic laccase for low-density polyethylene degradation. Journal of Hazardous Materials, 458: 131986. doi:10.1016/j.jhazmat.2023.131986.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000D-80ED-7 Version Permalink: https://hdl.handle.net/21.11116/0000-000E-3B9E-F

Genre: Journal Article

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https://doi.org/10.1016/j.jhazmat.2023.131986 (Publisher version) Open Access Hybrid

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Creators:
Zhang, Yan¹, Author
Plesner, Thea Jess¹, Author
Ouyang, Yi¹, Author
Zheng, Yu-Cong², Author
Bouhier, Etienne¹, Author
Berentzen, Emilie Ingemann¹, Author
Zhang, Mingliang¹, Author
Zhou, Pengfei¹, Author
Zimmermann, Wolfgang¹, Author
Andersen, Gregers Rom¹, Author
Eser, Bekir Engin¹, Author
Guo, Zheng¹, Author

Affiliations:
1external, ou_persistent22
2Max Planck Institute for Terrestrial Microbiology, Max Planck Society, Karl-von-Frisch-Strasse 10, D-35043 Marburg, DE, ou_3135468

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Free keywords: Enzyme discovery, Laccase, Thermophilic enzyme, PE degradation, Dye decolorization

Abstract: Polyethylene (PE) and industrial dyes are recalcitrant pollutants calling for the development of sustainable solutions for their degradation. Laccases have been explored for removal of contaminants and pollutants, including dye decolorization and plastic degradation. Here, a novel thermophilic laccase from PE-degrading Lysinibaccillus fusiformis (LfLAC3) was identified through a computer-aided and activity-based screening. Biochemical studies of LfLAC3 indicated its high robustness and catalytic promiscuity. Dye decolorization experiments showed that LfLAC3 was able to degrade all the tested dyes with decolorization percentage from 39% to 70% without the use of a mediator. LfLAC3 was also demonstrated to degrade low-density polyethylene (LDPE) films after eight weeks of incubation with either crude cell lysate or purified enzyme. The formation of a variety of functional groups was detected using Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS). Damage on the surfaces of PE films was observed via scanning electron microscopy (SEM). The potential catalytic mechanism of LfLAC3 was disclosed by structure and substrate-binding modes analysis. These findings demonstrated that LfLAC3 is a promiscuous enzyme that has promising potential for dye decolorization and PE degradation.

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Language(s): eng - English

Dates: Date issued: 2023

Publication Status: Issued

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Rev. Type: Peer

Identifiers: URI: https://www.sciencedirect.com/science/article/pii/S0304389423012694
DOI: 10.1016/j.jhazmat.2023.131986

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Title: Journal of Hazardous Materials

Other : J. Hazard. Mater.

Source Genre: Journal

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Publ. Info: Amsterdam : Elsevier

Pages: - Volume / Issue: 458 Sequence Number: 131986 Start / End Page: - Identifier: ISSN: 0304-3894
CoNE: https://pure.mpg.de/cone/journals/resource/954925512447