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  DNA origami signaling units transduce chemical and mechanical signals in synthetic cells

Jahnke, K., Illig, M., Scheffold, M., Tran, M. P., Mersdorf, U., & Göpfrich, K. (2023). DNA origami signaling units transduce chemical and mechanical signals in synthetic cells. Advanced Functional Materials, 2301176, pp. 1-10. doi:10.1002/adfm.202301176.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000D-83C4-1 Version Permalink: https://hdl.handle.net/21.11116/0000-000D-83C5-0
Genre: Journal Article

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 Creators:
Jahnke, Kevin1, Author           
Illig, Maja2, Author           
Scheffold, Marlene2, Author           
Tran, Mai P.2, Author           
Mersdorf, Ulrike3, Author           
Göpfrich, Kerstin1, Author           
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Max Planck Institute for Medical Research, Max Planck Society, ou_1125545              
3Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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Free keywords: giant unilamellar vesicle, DNA nanotechnology, DNA nanotubes, DNA pores, signal transduction, synthetic cells
 Abstract: Transmembrane proteins transmit chemical signals as well as mechanical cues. The latter is often achieved by coupling to the cytoskeleton. The incorporation of fully engineerable membrane-spanning structures for the transduction of chemical and, in particular, mechanical signals is therefore a critical aim for bottom-up synthetic biology. Here, a membrane-spanning DNA origami signaling units (DOSUs) is designed and mechanically coupled to DNA cytoskeletons encapsulated within giant unilamellar vesicles (GUVs). The incorporation of the DOSUs into the GUV membranes is verified and clustering upon external stimulation is achieved. Dye-influx assays reveal that clustering increases the insertion efficiency. The transmembrane-spanning DOSUs act as pores to allow for the transport of single-stranded DNA into the GUVs. This is employed to trigger the reconfiguration of DNA cytoskeletons within GUVs. In addition to chemical signaling, mechanical coupling of the DOSUs to the internal DNA cytoskeletons is induced. With chemical cues from the environment, clustering of the DOSUs is induced, which triggers a symmetry break in the organization of the DNA cytoskeleton inside of the GUV. DNA-based transmembrane structures are engineered that transduce signals without transporting the signaling molecule itself—providing a route toward signal processing and adaptive synthetic cells.

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Language(s): eng - English
 Dates: 2023-04-252023-02-012023-06-032023-06-03
 Publication Status: Published online
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1002/adfm.202301176
 Degree: -

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Title: Advanced Functional Materials
  Abbreviation : Adv. Funct. Mater.
Source Genre: Journal
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Publ. Info: Weinheim : Wiley-VCH Verlag GmbH
Pages: - Volume / Issue: - Sequence Number: 2301176 Start / End Page: 1 - 10 Identifier: ISSN: 1616-301X
CoNE: https://pure.mpg.de/cone/journals/resource/954925596563