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  Direct optical measurement of intra-molecular distances down to the Ångström scale

Sahl, S. J., Matthias, J., Inamdar, K., Khan, T. A., Weber, M., Becker, S., et al. (2023). Direct optical measurement of intra-molecular distances down to the Ångström scale. bioRxiv, 548133, pp. 1-12. doi:10.1101/2023.07.07.548133.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000D-8858-7 Version Permalink: https://hdl.handle.net/21.11116/0000-000D-8859-6
Genre: Journal Article

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 Creators:
Sahl, Steffen J., Author
Matthias, Jessica1, Author           
Inamdar, Kaushik, Author
Khan, Taukeer A., Author
Weber, Michael, Author
Becker, Stefan, Author
Griesinger, Christian, Author
Broichhagen, Johannes2, Author           
Hell, Stefan W.1, Author                 
Affiliations:
1Optical Nanoscopy, Max Planck Institute for Medical Research, Max Planck Society, ou_2364730              
2Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society, ou_2364732              

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 Abstract: Optical investigations of nanometer distances between proteins, their subunits, or other biomolecules have been the exclusive prerogative of Forster Resonance Energy Transfer (FRET) microscopy for decades. Here we show that MINFLUX fluorescence nanoscopy measures intra-molecular distances down to 1 nm - and in planar projections down to 1 Angstrom - directly, linearly, and with Angstrom precision. Our method is validated by quantifying well-characterized 1-to-10 nm distances in polypeptides and proteins. Moreover, we visualize the orientations of immunoglobulin subunits and reveal specific configurations of a histidine kinase domain dimer. Our results open the door for examining proximities and interactions of macromolecules under physiological conditions.

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Language(s): eng - English
 Dates: 2023-07-09
 Publication Status: Published online
 Pages: 12
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 Identifiers: DOI: 10.1101/2023.07.07.548133
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Title: bioRxiv
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Pages: - Volume / Issue: - Sequence Number: 548133 Start / End Page: 1 - 12 Identifier: -