English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
 Local TagsRelease HistoryDetailsSummary
  Chemical probe for imaging of polo-like Kinase 4 and centrioles

Salim, A., Werther, P., Hatzopoulos, G. N., Reymond, L., Wombacher, R., Gönczy, P., et al. (2023). Chemical probe for imaging of polo-like Kinase 4 and centrioles. JACS Au, 3(8), 2247-2256. doi:10.1021/jacsau.3c00271.

Item is

 

show all hide all

Basic

show hide
Item Permalink: https://hdl.handle.net/21.11116/0000-000D-8865-8 Version Permalink: https://hdl.handle.net/21.11116/0000-000D-ABCD-C
Genre: Journal Article

Files

show Files
hide Files
:
JACSAu_3_2023_2247.pdf (Any fulltext), 9MB
 
File Permalink:
-
Name:
JACSAu_3_2023_2247.pdf
Description:
-
OA-Status:
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
JACSAu_3_2023_2247_Suppl.pdf (Supplementary material), 6MB
 
File Permalink:
-
Name:
JACSAu_3_2023_2247_Suppl.pdf
Description:
-
OA-Status:
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Locator:
https://pubs.acs.org/doi/10.1021/jacsau.3c00271 (Any fulltext)
Description:
-
OA-Status:
Not specified
Locator:
https://pubs.acs.org/doi/epdf/10.1021/jacsau.3c00271 (Any fulltext)
Description:
-
OA-Status:
Not specified
Locator:
https://pubs.acs.org/doi/suppl/10.1021/jacsau.3c00271/suppl_file/au3c00271_si_001.pdf (Supplementary material)
Description:
-
OA-Status:
Not specified

Creators

show
hide
 Creators:
Salim, Aleksandar1, Author           
Werther, Philipp, Author
Hatzopoulos, Georgios N., Author
Reymond, Luc, Author
Wombacher, Richard1, Author           
Gönczy, Pierre, Author
Johnsson, Kai1, Author           
Affiliations:
1Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society, ou_2364732              

Content

show
hide
Free keywords: protein labeling; bio-orthogonal chemistry; fluorogenic fluorophores; Plk4; centriole imaging
 Abstract: Polo-like kinase (Plk4) is a serine/threonine-protein kinase that is essential for biogenesis of the centriole organelle and is enriched at centrioles. Herein, we introduce Cen-TCO, a chemical probe based on the Plk4 inhibitor centrinone, to image Plk4 and centrioles in live or fixed cultured human cells. Specifically, we established a bio-orthogonal two-step labeling system that enables the Cen-TCO-mediated imaging of Plk4 by STED super-resolution microscopy. Such direct labeling of Plk4 results in an increased resolution in STED imaging compared with using anti-Plk4 antibodies, underlining the importance of direct labeling strategies for super-resolution microscopy. We anticipate that Cen-TCO will become an important tool for investigating the biology of Plk4 and of centrioles.

Details

show
hide
Language(s): eng - English
 Dates: 2023-07-152023-05-302023-07-182023-08-042023-08-28
 Publication Status: Issued
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1021/jacsau.3c00271
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: JACS Au
  Abbreviation : JACS Au
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Washington, DC : American Chemical Society
Pages: - Volume / Issue: 3 (8) Sequence Number: - Start / End Page: 2247 - 2256 Identifier: ISSN: 2691-3704
CoNE: https://pure.mpg.de/cone/journals/resource/2691-3704