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Schlagwörter:
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Zusammenfassung:
Following application of mechanical lesions to the corpus cerebelli, a cerebellar subdivision, in adult individuals of the teleost fish Apteronotus leptorhynchus, the pattern of expression of the neuropeptide somatostatin was examined by employing immunohistochemical techniques. In the intact corpus cerebelli, only a very few cells displayed somatostatin-like immunoreactivity. This number dramatically increased in the area of the lesion within the granule cell layer 1 day following the injury and peaked after 2 days, when the normalized total number of somatostatin-positive cells was approximately 50 times higher than the mean number of labeled cells found after 3, 6, and 12 h of survival. Between 5 and 10 days of post-lesioning survival time, this number abruptly declined and returned to background levels at 17 and 25 days. Confocal microscopy revealed three cell types, presumably corresponding to granule cell neurons, astrocytes and microglia, which all displayed a similar temporal pattern of somatostatin expression. It is hypothesized that somatostatin is involved in regulation of the genesis and/or development of new neurons which are produced in response to injuries and which replace damaged cells at the site of the lesion.
