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  Bioorthogonal caging-group-free photoactivatable probes for minimal-linkage-error nanoscopy

Aktalay, A., Lincoln, R., Heynck, L., Lima, M. A. d. R. B. F., Butkevich, A. N., Bossi, M. L., et al. (2023). Bioorthogonal caging-group-free photoactivatable probes for minimal-linkage-error nanoscopy. ACS Central Science, 9(8), 1581-1590. doi:10.1021/acscentsci.3c00746.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000D-9384-7 Version Permalink: https://hdl.handle.net/21.11116/0000-000D-A2CF-3
Genre: Journal Article

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https://pubs.acs.org/doi/10.1021/acscentsci.3c00746 (Any fulltext)
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 Creators:
Aktalay, Ayse1, Author           
Lincoln, Richard1, Author           
Heynck, Lukas1, Author           
Lima, Maria Augusta do R. B. F.1, Author           
Butkevich, Alexey N.1, Author           
Bossi, Mariano L.1, Author           
Hell, Stefan W.1, Author                 
Affiliations:
1Optical Nanoscopy, Max Planck Institute for Medical Research, Max Planck Society, ou_2364730              

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 Abstract: Here we describe highly compact, click compatible, and photoactivatable dyes for super-resolution fluorescence microscopy (nanoscopy). By combining the photoactivatable xanthone (PaX) core with a tetrazine group, we achieve minimally sized and highly sensitive molecular dyads for the selective labeling of unnatural amino acids introduced by genetic code expansion. We exploit the excited state quenching properties of the tetrazine group to attenuate the photoactivation rates of the PaX, and further reduce the overall fluorescence emission of the photogenerated fluorophore, providing two mechanisms of selectivity to reduce the off-target signal. Coupled with MINFLUX nanoscopy, we employ our dyads in the minimal-linkage-error imaging of vimentin filaments, demonstrating molecular-scale precision in fluorophore positioning.

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Language(s): eng - English
 Dates: 2023-06-202023-07-262023-08-23
 Publication Status: Issued
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1021/acscentsci.3c00746
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Title: ACS Central Science
  Other : ACS Cent. Sci.
Source Genre: Journal
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Publ. Info: Washington : American Chemical Society
Pages: - Volume / Issue: 9 (8) Sequence Number: - Start / End Page: 1581 - 1590 Identifier: Other: 2374-7951
CoNE: https://pure.mpg.de/cone/journals/resource/2374-7951