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  Divergent combinations of cis-regulatory elements control the evolution of phenotypic plasticity

Dardiry, M., Eberhardt, G., Witte, H., Rödelsperger, C., Rödelsperger, C., Lightfoot, J. W., et al. (2023). Divergent combinations of cis-regulatory elements control the evolution of phenotypic plasticity. PLoS Biology, 21(8): e3002270. doi:10.1371/journal.pbio.3002270.

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2023
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© 2023 Dardiry et al.

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https://www.ebi.ac.uk/ena/browser/view/PRJEB59264 (Supplementary material)
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RNA-seq data has been deposited at the European Nucleotide Archive under the study accession PRJEB59264 and PRJEB13695.
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https://www.ebi.ac.uk/ena/browser/view/PRJEB13695 (Supplementary material)
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RNA-seq data has been deposited at the European Nucleotide Archive under the study accession PRJEB59264 and PRJEB13695.
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 Creators:
Dardiry, M1, Author                 
Eberhardt, G1, Author                 
Witte, H1, Author                 
Rödelsperger, C1, Author                 
Rödelsperger, Christian1, Author                 
Lightfoot, James W2, Author                 
Sommer, RJ1, Author                 
Affiliations:
1Department Integrative Evolutionary Biology, Max Planck Institute for Biology Tübingen, Max Planck Society, ou_3371685              
2Max Planck Research Group Genetics of Behavior, Max Planck Institute for Neurobiology of Behavior – caesar, Max Planck Society, ou_3361790              

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 Abstract: The widespread occurrence of phenotypic plasticity across all domains of life demonstrates its evolutionary significance. However, how plasticity itself evolves and how it contributes to evolution is poorly understood. Here, we investigate the predatory nematode Pristionchus pacificus with its feeding structure plasticity using recombinant-inbred-line and quantitative-trait-locus (QTL) analyses between natural isolates. We show that a single QTL at a core developmental gene controls the expression of the cannibalistic morph. This QTL is composed of several cis-regulatory elements. Through CRISPR/Cas-9 engineering, we identify copy number variation of potential transcription factor binding sites that interacts with a single intronic nucleotide polymorphism. Another intronic element eliminates gene expression altogether, mimicking knockouts of the locus. Comparisons of additional isolates further support the rapid evolution of these cis-regulatory elements. Finally, an independent QTL study reveals evidence for parallel evolution at the same locus. Thus, combinations of cis-regulatory elements shape plastic trait expression and control nematode cannibalism.

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Language(s): eng - English
 Dates: 2023-08
 Publication Status: Published online
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1371/journal.pbio.3002270
PMID: 37590316
 Degree: -

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Title: PLoS Biology
  Abbreviation : PLoS Biol
Source Genre: Journal
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Publ. Info: San Francisco, California, US : Public Library of Science
Pages: - Volume / Issue: 21 (8) Sequence Number: e3002270 Start / End Page: - Identifier: ISSN: 1544-9173
CoNE: https://pure.mpg.de/cone/journals/resource/111056649444170