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  Simplified Preservation of Equivalent Pathways Spectroscopy

Nimerovsky, E., Varkey, A. C., Kim, M., Becker, S., & Andreas, L. B. (2023). Simplified Preservation of Equivalent Pathways Spectroscopy. JACS Au, 3(10), 2763-2771. doi:10.1021/jacsau.3c00312.

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 Creators:
Nimerovsky, Evgeny1, 2, Author           
Varkey, Abel Cherian1, 2, Author           
Kim, Myeongkyu1, 2, Author           
Becker, Stefan1, 2, Author           
Andreas, Loren B.1, 2, Author           
Affiliations:
1Department of NMR Based Structural Biology, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society, ou_3350124              
2Research Group of Solid State NMR Spectroscopy-2, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society, ou_3350125              

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 Abstract: Inspired by the recently proposed transverse mixing optimal control pulses (TROP) approach for improving signal in multidimensional magic-angle spinning (MAS) NMR experiments, we present simplified preservation of equivalent pathways spectroscopy (SPEPS). It transfers both transverse components of magnetization that occur during indirect evolutions, theoretically enabling a √2 improvement in sensitivity for each such dimension. We compare SPEPS transfer with TROP and cross-polarization (CP) using membrane protein and fibril samples at MAS of 55 and 100 kHz. In three-dimensional (3D) (H)CANH spectra, SPEPS outperformed TROP and CP by factors of on average 1.16 and 1.69, respectively, for the membrane protein, but only a marginal improvement of 1.09 was observed for the fibril. These differences are discussed, making note of the longer transfer time used for CP, 14 ms, as compared with 2.9 and 3.6 ms for SPEPS and TROP, respectively. Using SPEPS for two transfers in the 3D (H)CANCO experiment resulted in an even larger benefit in signal intensity, with an average improvement of 1.82 as compared with CP. This results in multifold time savings, in particular considering the weaker peaks that are observed to benefit the most from SPEPS.

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Language(s): eng - English
 Dates: 2023-10-112023-10-23
 Publication Status: Issued
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 Rev. Type: Peer
 Identifiers: DOI: 10.1021/jacsau.3c00312
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Title: JACS Au
  Abbreviation : JACS Au
Source Genre: Journal
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Publ. Info: Washington, DC : American Chemical Society
Pages: - Volume / Issue: 3 (10) Sequence Number: - Start / End Page: 2763 - 2771 Identifier: ISSN: 2691-3704
CoNE: https://pure.mpg.de/cone/journals/resource/2691-3704