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  Photoactivatable carbo- and silicon-rhodamines and their application in MINFLUX nanoscopy

Aktalay, A., Khan, T. A., Bossi, M. L., Belov, V. N., & Hell, S. W. (2023). Photoactivatable carbo- and silicon-rhodamines and their application in MINFLUX nanoscopy. Angewandte Chemie International Edition, 62(14): e202302781, pp. 1-9. doi:10.1002/anie.202302781.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000D-A9B2-B Version Permalink: https://hdl.handle.net/21.11116/0000-000D-C494-E
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 Creators:
Aktalay, Ayse1, Author           
Khan, Taukeer A., Author
Bossi, Mariano L.1, Author           
Belov, Vladimir N., Author
Hell, Stefan W.1, Author                 
Affiliations:
1Optical Nanoscopy, Max Planck Institute for Medical Research, Max Planck Society, ou_2364730              

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Free keywords: Dyes/Pigments · Fluorescence · Photolysis · Single-Molecule Photoactivation · Superresolution Microscopy
 Abstract: New photoactivatable fluorescent dyes (rhodamine, carbo- and silicon-rhodamines [SiR]) with emission ranging from green to far red have been prepared, and their photophysical properties studied. The photocleavable 2-nitrobenzyloxycarbonyl unit with an alpha-carboxyl group as a branching point and additional functionality was attached to a polycyclic and lipophilic fluorescent dye. The photoactivatable probes having the HaloTagTM amine (O2) ligand bound with a dye core were obtained and applied for live-cell staining in stable cell lines incorporating Vimentin (VIM) or Nuclear Pore Complex Protein NUP96 fused with the HaloTag. The probes were applied in 2D (VIM, NUP96) and 3D (VIM) MINFLUX nanoscopy, as well as in superresolution fluorescence microscopy with single fluorophore activation (VIM, live-cell labeling). Images of VIM and NUPs labeled with different dyes were acquired and their apparent dimensions and shapes assessed on a lower single-digit nanometer scale. Applicability and performance of the photoactivatable dye derivatives were evaluated in terms of photoactivation rate, labeling and detection efficiency, number of detected photons per molecule and other parameters related to MINFLUX nanoscopy.

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Language(s): eng - English
 Dates: 2023-02-232023-08-092023-09-01
 Publication Status: Issued
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1002/anie.202302781
 Degree: -

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Title: Angewandte Chemie International Edition
  Abbreviation : Angew. Chem. Int. Ed.
Source Genre: Journal
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Publ. Info: Weinheim : Wiley-VCH
Pages: - Volume / Issue: 62 (14) Sequence Number: e202302781 Start / End Page: 1 - 9 Identifier: ISSN: 1433-7851
CoNE: https://pure.mpg.de/cone/journals/resource/1433-7851