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  Simultaneous Behavioral and Neuronal Imaging by Tracking Microscopy

Robson, D., & Li, J. (2023). Simultaneous Behavioral and Neuronal Imaging by Tracking Microscopy. In J. Amatruda, C. Houart, K. Kawakami, & K. Poss (Eds.), Zebrafish: Methods and Protocols (pp. 155-167). New York, NY, USA: Humana Press.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000D-ABF7-C Version Permalink: https://hdl.handle.net/21.11116/0000-000D-ABF8-B
Genre: Book Chapter

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https://link.springer.com/content/pdf/10.1007/978-1-0716-3401-1_10.pdf (Publisher version)
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 Creators:
Robson, DN1, Author                 
Li, JM1, Author                 
Affiliations:
1Research Group Systems Neuroscience & Neuroengineering, Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_3168483              

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 Abstract: Tracking microscopy enables whole-brain cellular resolution imaging in freely swimming animals. This technique enables both structural and functional imaging without immobilizing the animal, and greatly expands the range of the behaviors accessible to neuroscientists. We use infrared imaging to track the target animal in a behavioral arena. Based on the predicted trajectory of the brain, we apply optimal control theory to a motorized stage system to cancel brain motion in three dimensions. We have combined this motion cancellation system with Differential Illumination Focal Filtering (DIFF), a form of structured illumination microscopy, which enables us to image the brain of a freely swimming larval zebrafish for over an hour. Here we describe the typical experimental procedure for data acquisition and processing using the tracking microscope.

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 Dates: 2023-10
 Publication Status: Issued
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1007/978-1-0716-3401-1_10
 Degree: -

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Title: Zebrafish: Methods and Protocols
Source Genre: Book
 Creator(s):
Amatruda, JF, Editor
Houart, C, Editor
Kawakami, K, Editor
Poss, KD, Editor
Affiliations:
-
Publ. Info: New York, NY, USA : Humana Press
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 155 - 167 Identifier: ISBN: 978-1-0716-3400-4
DOI: 10.1007/978-1-0716-3401-1

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Title: Methods in Molecular Biology
  Other : Methods Mol. Biol.
Source Genre: Series
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Publ. Info: US : Springer
Pages: - Volume / Issue: 2707 Sequence Number: - Start / End Page: - Identifier: ISSN: 1064-3745
CoNE: https://pure.mpg.de/cone/journals/resource/954927725544