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  Novel insights into the regulation of transgenerational epigenetic inheritance in Pristionchus pacificus

Quiobe, S., Witte, H., Rödelsperger, C., & Sommer, R. (2023). Novel insights into the regulation of transgenerational epigenetic inheritance in Pristionchus pacificus. In 4th International Pristonchus Meeting (pp. 35).

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アイテムのパーマリンク: https://hdl.handle.net/21.11116/0000-000D-B498-C 版のパーマリンク: https://hdl.handle.net/21.11116/0000-000F-B9F8-9
資料種別: 会議抄録

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 作成者:
Quiobe, SP1, 著者                 
Witte, H1, 著者                 
Rödelsperger, C1, 2, 著者                 
Sommer, RJ1, 著者                 
所属:
1Department Integrative Evolutionary Biology, Max Planck Institute for Biology Tübingen, Max Planck Society, Max-Planck-Ring 5, 72076 Tübingen, ou_3371685              
2Evolutionary Genomics and Bioinformatics Group, Department Integrative Evolutionary Biology, Max Planck Institute for Biology Tübingen, Max Planck Society, ou_3507717              

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 要旨: Environmental cues can have profound effects on organismal development and phenotype, often regulated by small RNAs. Using long-term environmental induction approaches, we aim to study the influence of shifts in microbial diet on mouth-form plasticity in Pristionchus pacificus. Utilizing the rapidly propagating isogenic populations of P. pacificus with its direct binary mouth-form phenotype, directed evolution for 101 generations on a Novosphingobium diet revealed immediate and systemic diet- induced plasticity. Periodic diet-reversals to OP50 revealed memories that are not limited to the first- generation offspring but maintained transgenerationally. We combined these long-term induction experiments with unbiased forward genetic screens by performing an EMS mutagenesis in generation F14 on Novosphingobium to find mutants defective in transgenerational inheritance. From a screen of 9,900 ‘F2’ progeny of mutagenized animals, we found 165 potential candidates for transgenerational inheritance defective (tid) after food reversal. Whole genome sequencing revealed that multiple candidate genes were hit several times independently (i.e. >3 alleles). Indeed, generating clean CRISPR mutants in the first four candidate genes revealed a tid phenotype after diet reversal. Providing novel insight into transgenerational inheritance, we will describe novel molecular mechanisms involving sets of epigenetic factors identified through the analysis of these tid mutants.

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 日付: 2023-09
 出版の状態: オンラインで出版済み
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関連イベント

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イベント名: 4th International Pristonchus Meeting 2023
開催地: Tübingen, Germany
開始日・終了日: 2023-09-22 - 2023-09-23

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出版物名: 4th International Pristonchus Meeting
種別: 会議論文集
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ページ: - 巻号: - 通巻号: T21 開始・終了ページ: 35 識別子(ISBN, ISSN, DOIなど): -