ausblenden:
Schlagwörter:
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Zusammenfassung:
In C. elegans, upon cleavage of an RNA by the RNAi or the piRNA mechanisms, a poly(UG) tail is added to the cleaved RNA by RDE-3. This tailed RNA serves as template for the transcription of secondary siRNAs by RNA dependent RNA polymerases. Injection of such tailed RNAs leads to gene silencing. Upon injection of tailed RNAs into P. pacificus, we also observed gene knock down and the formation of new tailed RNAs with the endogenous and the injected RNAs used as substrates. Contrary to C. elegans, in P. pacificus the rde-3 mutation is maternal effect sterile. We also injected UG tailed RNAs into the clade IV parasitic nematode Strongyloides stercoralis but so far failed to detect the formation of new tailed RNAs. In the second part of my poster, I will show the results of my recent sampling trip to Bangladesh. Compared with earlier studies in South East Asia, we found a rather high incidence of Strongyloides fuelleborni, a species normally considered to occur only very rarely in humans as a zoonotic infection from monkeys and we found, for the first time in a human, a genetic type of S. stercoralis that had been described as restricted to dogs.
