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Zusammenfassung:
Developmental plasticity is widespread in a range of organisms from bacteria, plants to animals, and can be triggered by changing environmental conditions. Phenotypic plasticity in Pristionchus pacificus is seen in the form of dietary mouth-form dimorphism. P. pacificus (RSC011), which is 30% eurystomatous on OP50, shows complete drastic turnover in mouth form to (predatory) eurystomatous on Novospingobium diet. Long term environmental induction (LTEI) on Novospingobium resulted in immediate, systemic and complete effect for 101 generations. Furthermore, diet switch (reversal) experiments to OP50 showed substantial and evident transgenerational epigenetic inheritance (TEI) effect. Unbiased genetic screens revealed importance of two novel and important genes, Ppa-sut-2 and Ppa-pgp-11. Our aim is to comprehend the role of these genes involved in this TEI mechanism by observing their protein expression localization and interacting partners. Downstream RNA-seq analysis of Ppa-sut-2 mutant showed role of worm specific argonauts, Ppa-wago-1 and Ppa-wago-10, in TEI. TEI-test of Ppa-wago-1 and Ppa-wago-10 mutants also showed TEI defective effect post reversal on OP50 diet. In this study, we showed, yet again, the importance of Ppa-WAGOs in TEI transmission.
