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Schlagwörter:
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Zusammenfassung:
miR-100 is a highly conserved microRNA, found across different bilaterian species, and possibly even originating from the common ancestor of eumetazoans1 . As such, its absence in the C. elegans genome is intriguing2 . We identify miR-100 as one of the most abundant microRNAs in juvenile stages of P. pacificus (Ppc-miR-100). Interestingly, P. pacificus genome shows a partial conservation of the polycistronic let-7-complex3 , found in multiple species except C. elegans2 , wherein Ppc-miR-100 is found approximately 200 bps upstream of Ppc-let-7, but does not contain Ppc-lin-4 in the same locus. Knock-out of Ppc-miR-100 results in mild but consistent germline defects, a developmental disturbance that is exacerbated in the Ppc-let-7 mutant. Interestingly, Ppc-lin-4 mutants show near normal overall development. Our study provides deeper insights into the field of small RNA biology and molecular evolution, furthering our understanding of the roles of microRNAs in development and nematode evolution.
