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  Staphylococcus aureus Endoribonuclease III: Purification and Properties

Chevalier, C., Huntzinger, E., Fechter, P., Boisset, S., Vandenesch, V., Romby, P., et al. (2008). Staphylococcus aureus Endoribonuclease III: Purification and Properties. In L. Maquat, & C. Arraiano (Eds.), RNA Turnover in Bacteria, Archaea and Organelles (pp. 309-327). Amsterdam, The Netherlands: Elsevier Academic Press.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000D-B55B-1 Version Permalink: https://hdl.handle.net/21.11116/0000-000D-B55C-0
Genre: Book Chapter

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 Creators:
Chevalier, C, Author
Huntzinger, E1, Author                 
Fechter, P, Author
Boisset, S, Author
Vandenesch, V, Author
Romby, P, Author
Geissmann, T, Author
Affiliations:
1Department Biochemistry, Max Planck Institute for Developmental Biology, Max Planck Society, ou_3375718              

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 Abstract: Staphylococcus aureus ribonuclease III (Sa‐RNase III) belongs to the enzyme family known to process double‐stranded RNAs consisting of two turns of the RNA helix. Although the enzyme is thought to play a role in ribosomal RNA processing and gene regulation, the deletion of the rnc gene in S. aureus does not affect cell growth in rich medium. S. aureus RNase III acts in concert with regulatory RNAIII to repress the expression of several mRNAs encoding virulence factors. The action of the RNase is most likely to initiate the degradation of repressed mRNAs leading to an irreversible repression. In this chapter, we describe the overexpression and purification of recombinant RNase III from S. aureus, and we show that its biochemical properties are similar to the orthologous enzyme from Escherichia coli. Both enzymes similarly recognize and cleave different RNA substrates and RNA‐mRNA duplexes.

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 Dates: 2008
 Publication Status: Issued
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Title: RNA Turnover in Bacteria, Archaea and Organelles
Source Genre: Book
 Creator(s):
Maquat, LE, Editor
Arraiano, CM, Editor
Affiliations:
-
Publ. Info: Amsterdam, The Netherlands : Elsevier Academic Press
Pages: - Volume / Issue: - Sequence Number: 16 Start / End Page: 309 - 327 Identifier: ISBN: 978-0-12-374377-0

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Title: Methods in Enzymology
  Other : Methods Enzymol.
Source Genre: Series
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Publ. Info: New York, NY : Academic Press
Pages: - Volume / Issue: 447 Sequence Number: - Start / End Page: - Identifier: ISSN: 0076-6879
CoNE: https://pure.mpg.de/cone/journals/resource/110975506069301