Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
  The importance of cis-regulatory variation in regulatory and gene-expression divergence of stickleback ecotypes

Neufeld, S., Verta, J.-P., Wang, M., Schwickert, J., John, M., Ritter, M., et al. (2021). The importance of cis-regulatory variation in regulatory and gene-expression divergence of stickleback ecotypes. In SMBE 2021 (pp. 486).

Item is

Basisdaten

einblenden: ausblenden:
Genre: Meeting Abstract

Externe Referenzen

einblenden:
ausblenden:
Beschreibung:
-
OA-Status:
Keine Angabe

Urheber

einblenden:
ausblenden:
 Urheber:
Neufeld, S1, Autor           
Verta, J-P1, Autor                 
Wang, M1, Autor           
Schwickert, J1, Autor           
John, M1, Autor                 
Ritter, M1, Autor           
Scionti, D1, Autor                 
Kick, S1, Autor           
Kirch, M1, Autor                 
Avdievich, E1, Autor           
Venu, V1, Autor                 
Jones, FC1, Autor                 
Affiliations:
1Jones Group, Friedrich Miescher Laboratory, Max Planck Society, ou_3008689              

Inhalt

einblenden:
ausblenden:
Schlagwörter: -
 Zusammenfassung: The adaptation of natural populations to changing environments is often driven by numerous genetic loci predominantly found in non-coding regions with likely gene regulatory roles. Using adaptively diverging marine and freshwater stickleback fish ecotypes as a model, we quantified the degree to which cis- and trans- acting factors underlie expression divergence with allele-specific gene expression assays. Finding a major role for cis-regulatory differences compared to trans-regulatory differences, we next performed comparative epigenomics, chromatin profiling, transcriptomics and genetics to identify thousands of cis- regulatory elements with divergent epigenomic profiles between the ecotypes. Divergent elements are enriched at the promoter and 5’UTR of genes, are proximal to genes showing differential expression, and vary across tissues, with the liver showing considerably higher regulatory divergence than kidney or gills. Allele-specific analyses in F1 hybrids reveals that divergence in chromatin accessibility is itself mostly cis-regulated and these elements show molecular signatures of natural selection. Additionally, forward genetic mapping of chromatin variation identifies QTLs located on the same chromosome as each of the divergent chromatin peaks, and finds little evidence for trans-acting factors controlling differences in chromatin accessibility and regulatory genome function. The high resolution maps of the chromatin and epigenomic landscape in diverging stickleback ecotypes provides functional annotation of regulatory elements within adaptive loci. Combined, our studies show overwhelming evidence for the major role of cis-regulation of the regulatory genome and gene expression in the adaptive divergence of marine and freshwater stickleback in the early stages of speciation.

Details

einblenden:
ausblenden:
Sprache(n):
 Datum: 2021-07
 Publikationsstatus: Online veröffentlicht
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: -
 Art des Abschluß: -

Veranstaltung

einblenden:
ausblenden:
Titel: Society for Molecular Biology and Evolution Conference 2021 (SMBE 2021)
Veranstaltungsort: -
Start-/Enddatum: 2019-07-03 - 2019-07-08

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: SMBE 2021
Genre der Quelle: Konferenzband
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: - Artikelnummer: SYMP4-5 Start- / Endseite: 486 Identifikator: -