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  Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila

Pérez-Mojica, J. E., Enders, L., Lau, K. H., & Lempradl, A. (2023). Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila. STAR Protocols, 4: 102535. doi:10.1016/j.xpro.2023.102535.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000D-B6F3-3 Version Permalink: https://hdl.handle.net/21.11116/0000-000D-B6F4-2
Genre: Journal Article

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10.1016_j.it.2023.01.004.pdf (Publisher version), 6MB
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2023
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The Author(s). Published by Elsevier Inc. All rights reserved.
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https://www.sciencedirect.com/science/article/pii/S2666166723005026?via%3Dihub (Publisher version)
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 Creators:
Pérez-Mojica, J Eduardo1, Author
Enders, Lennart2, Author
Lau, Kin H1, Author
Lempradl, Adelheid1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Department of Epigenetics, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243644              

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Free keywords: Developmental Biology; Gene Expression; RNAseq
 Abstract: Exploring early embryonic gene expression is challenging due to the rate of development and the limited material available. Here, we present a protocol for ordering Drosophila embryos along a developmental pseudo-time trajectory and determining the sex of the embryos using RNA-seq data. We describe steps for sample collection, RNA isolation, RNA-seq, and RNA-seq data processing. We then detail the establishment of a continuous transcriptome dataset for assessing gene expression throughout early development and in a sex-specific manner. For complete details on the use and execution of this protocol, please refer to Pérez-Mojica et al.1.

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Language(s): eng - English
 Dates: 2023-09-15
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.xpro.2023.102535
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Title: STAR Protocols
Source Genre: Journal
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Publ. Info: Cambridge, MA ; Amsterdam : Cell Press ; Elsevier
Pages: - Volume / Issue: 4 Sequence Number: 102535 Start / End Page: - Identifier: ISSN: 2666-1667
CoNE: https://pure.mpg.de/cone/journals/resource/2666-1667