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  Ion selectivity and rotor coupling of the Vibrio flagellar sodium-driven stator unit.

Hu, H., Popp, P. F., Santiveri, M., Roa-Eguiara, A., Yan, Y., Martin, F. J. O., et al. (2023). Ion selectivity and rotor coupling of the Vibrio flagellar sodium-driven stator unit. Nature communications, 14(1), 4411. doi:10.1038/s41467-023-39899-z.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000D-CACD-9 Version Permalink: https://hdl.handle.net/21.11116/0000-000D-CACE-8
Genre: Journal Article

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 Creators:
Hu, Haidai, Author
Popp, Philipp F., Author
Santiveri, Mònica, Author
Roa-Eguiara, Aritz, Author
Yan, Yumeng, Author
Martin, Freddie J. O., Author
Liu, Zheyi, Author
Wadhwa, Navish, Author
Wang, Yong, Author
Erhardt, Marc1, Author
Taylor, Nicholas M. I., Author
Affiliations:
1Max Planck Unit for the Science of Pathogens, Max Planck Society, ou_3213696              

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Free keywords: *Bacterial Proteins/metabolism, *Sodium/metabolism, Cryoelectron Microscopy, Flagella/metabolism, Humans, Molecular Motor Proteins/metabolism, Vibrio alginolyticus/chemistry/metabolism
 Abstract: Bacteria swim using a flagellar motor that is powered by stator units. Vibrio spp. are highly motile bacteria responsible for various human diseases, the polar flagella of which are exclusively driven by sodium-dependent stator units (PomAB). However, how ion selectivity is attained, how ion transport triggers the directional rotation of the stator unit, and how the stator unit is incorporated into the flagellar rotor remained largely unclear. Here, we have determined by cryo-electron microscopy the structure of Vibrio PomAB. The electrostatic potential map uncovers sodium binding sites, which together with functional experiments and molecular dynamics simulations, reveal a mechanism for ion translocation and selectivity. Bulky hydrophobic residues from PomA prime PomA for clockwise rotation. We propose that a dynamic helical motif in PomA regulates the distance between PomA subunit cytoplasmic domains, stator unit activation, and torque transmission. Together, our study provides mechanistic insights for understanding ion selectivity and rotor incorporation of the stator unit of the bacterial flagellum.

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 Dates: 2023-07
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1038/s41467-023-39899-z
BibTex Citekey: hu_ion_2023
 Degree: -

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Title: Nature communications
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 14 (1) Sequence Number: - Start / End Page: 4411 Identifier: ISSN: 2041-1723