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  Imaging proteins at the single-molecule level

Longchamp, J., Rauschenbach, S., Abb, S., Escher, C., Latychevskaia, T., Kern, K., et al. (2017). Imaging proteins at the single-molecule level. Proceedings of the National Academy of Sciences of the United States of America, 114(7), 1474-1479.

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 Creators:
Longchamp, J., Author
Rauschenbach, S.1, Author           
Abb, S., Author
Escher, C., Author
Latychevskaia, T., Author
Kern, K.1, Author           
Fink, H., Author
Affiliations:
1Department Nanoscale Science (Klaus Kern), Max Planck Institute for Solid State Research, Max Planck Society, ou_3370481              

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Free keywords: low-energy electron holography; single protein imaging; preparative mass spectrometry; microscopy; structural biology
 Abstract: Imaging single proteins has been a long-standing ambition for advancing various fields in natural science, as for instance structural biology, biophysics, and molecular nanotechnology. In particular, revealing the distinct conformations of an individual protein is of utmost importance. Here, we show the imaging of individual proteins and protein complexes by low-energy electron holography. Samples of individual proteins and protein complexes on ultraclean freestanding graphene were prepared by soft-landing electrospray ion beam deposition, which allows chemical- and conformational-specific selection and gentle deposition. Low-energy electrons do not induce radiation damage, which enables acquiring subnanometer resolution images of individual proteins (cytochrome C and BSA) as well as of protein complexes (hemoglobin), which are not the result of an averaging process.

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Language(s): eng - English
 Dates: 2017
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 734859
ISI: 000393989300042
 Degree: -

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Title: Proceedings of the National Academy of Sciences of the United States of America
Source Genre: Journal
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Affiliations:
Publ. Info: WASHINGTON : NATL ACAD SCIENCES
Pages: - Volume / Issue: 114 (7) Sequence Number: - Start / End Page: 1474 - 1479 Identifier: ISSN: 0027-8424