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  A miniTurbo-based proximity labeling protocol to identify conditional protein interactomes in vivo in Myxococcus xanthus

Herfurth, M., Müller, F., Søgaard-Andersen, L., & Glatter, T. (2023). A miniTurbo-based proximity labeling protocol to identify conditional protein interactomes in vivo in Myxococcus xanthus. STAR Protocols, 4(4): 102657. doi:10.1016/j.xpro.2023.102657.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000D-D87B-6 Version Permalink: https://hdl.handle.net/21.11116/0000-000F-2043-1
Genre: Journal Article

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Locator:
https://doi.org/10.1016/j.xpro.2023.102657 (Publisher version)
Description:
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OA-Status:
Gold

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 Creators:
Herfurth, Marco1, Author           
Müller, Franziska1, Author           
Søgaard-Andersen, Lotte1, Author                 
Glatter, Timo2, Author                 
Affiliations:
1Bacterial Adaption and Differentiation, Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266305              
2Core Facility Mass Spectrometry and Proteomics, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266266              

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Free keywords: Microbiology, Proteomics, Mass Spectrometry
 Abstract: Summary
Protein-protein interactions are foundational for many cellular processes. Such interactions are especially challenging to identify if they are transient or depend on environmental conditions. This protocol details steps to identify stable and transient protein interactomes in the bacterium Myxococcus xanthus using biotin ligase miniTurbo-based proximity labeling. We include instructions for optimizing the expression of control proteins, in vivo biotin labeling of bacteria grown on a surface or in suspension culture, enrichment of biotinylated proteins, and sample processing for proteomic analysis. For complete details on the use and execution of this protocol, please refer to Branon et al. (2018).1

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Language(s): eng - English
 Dates: 2023-12-15
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: URI: https://www.sciencedirect.com/science/article/pii/S266616672300624X
DOI: 10.1016/j.xpro.2023.102657
 Degree: -

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Funding organization : Max Planck Society

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Title: STAR Protocols
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Cambridge, MA ; Amsterdam : Cell Press ; Elsevier
Pages: - Volume / Issue: 4 (4) Sequence Number: 102657 Start / End Page: - Identifier: ISSN: 2666-1667
CoNE: https://pure.mpg.de/cone/journals/resource/2666-1667