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  Multi-color super-resolution imaging to study human coronavirus RNA during cellular infection

Wang, J., Han, M., Roy, A. R., Wang, H., Möckl, L., Zeng, L., et al. (2022). Multi-color super-resolution imaging to study human coronavirus RNA during cellular infection. Cell Reports Methods, 2(2). doi:10.1016/j.crmeth.2022.100170.

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PIIS2667237522000224.pdf (Publisher version), 6MB
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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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 Creators:
Wang, Jiarui1, Author
Han, Mengting1, Author
Roy, Anish R.1, Author
Wang, Haifeng1, Author
Möckl, Leonhard2, 3, Author           
Zeng, Leiping1, Author
Moerner, W.E.1, Author
Qi, Lei S.1, Author
Affiliations:
1external, ou_persistent22              
2Möckl Research Group, Research Groups, Max Planck Institute for the Science of Light, Max Planck Society, ou_3263639              
3Department of Chemistry, Stanford University, Stanford, CA 94305, USA, ou_persistent22              

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 Abstract: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the third human coronavirus within 20 years that gave rise to a life-threatening disease and the first to reach pandemic spread. To make therapeutic headway against current and future coronaviruses, the biology of coronavirus RNA during infection must be precisely understood. Here, we present a robust and generalizable framework combining high-throughput confocal and super-resolution microscopy imaging to study coronavirus infection at the nanoscale. Using the model human coronavirus HCoV-229E, we specifically labeled coronavirus genomic RNA (gRNA) and double-stranded RNA (dsRNA) via multi-color RNA immunoFISH and visualized their localization patterns within the cell. The 10-nm resolution achieved by our approach uncovers a striking spatial organization of gRNA and dsRNA into three distinct structures and enables quantitative characterization of the status of the infection after antiviral drug treatment. Our approach provides a comprehensive imaging framework that will enable future investigations of coronavirus fundamental biology and therapeutic effects.

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Language(s): eng - English
 Dates: 2022-02-28
 Publication Status: Issued
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 Identifiers: DOI: 10.1016/j.crmeth.2022.100170
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Title: Cell Reports Methods
  Other : Cell Reports: Methods
Source Genre: Journal
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Publ. Info: Amsterdam, The Netherlands : Elsevier
Pages: - Volume / Issue: 2 (2) Sequence Number: - Start / End Page: - Identifier: Other: ISSN
CoNE: https://pure.mpg.de/cone/journals/resource/2667-2375