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  An investigation into the potential of peptide mass fingerprinting for the study of Australian faunal assemblages

Peters, C. (2022). An investigation into the potential of peptide mass fingerprinting for the study of Australian faunal assemblages. PhD Thesis, Friedrich-Schiller-Universität Jena, Philosophische Fakultät, Jena. Retrieved from http://uri.gbv.de/document/gvk:ppn:1852851171.

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Dissertation, Friedrich-Schiller-Universität Jena, 2023
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phd thesis for free download (Any fulltext)
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 Creators:
Peters, Carli1, Author           
Boivin, Nicole L.1, Referee           
Affiliations:
1Archaeology, Max Planck Institute for the Science of Human History, Max Planck Society, ou_2074312              

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Free keywords: Australien <Motiv>, Tierknochenfund, Archäozoologie, Paläozoologie, Massenspektrometrie, Peptide, Fingerprint-Verfahren
 Abstract: One of the main challenges to the study of past fauna is the often high level of fragmentation of bone assemblages. Zooarchaeology by Mass Spectrometry (ZooMS) is a biomolecular method that can be used to identify fragmented bones on the basis of their unique collagen fingerprints. So far, ZooMS studies have mostly focused on Eurasian contexts, but the method also has significant potential for the study of faunal assemblages in other regions of the world. This thesis explores the potential of Zooarchaeology by Mass Spectrometry (ZooMS) to study Australian faunal assemblages. Two major challenges limiting ZooMS applications in Australia are addressed: the lack of a reference database, and limited knowledge of collagen preservation in the country. Then, the potential of ZooMS to study Australian faunal assemblages is further explored.

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Language(s): eng - English
 Dates: 2023-07-182022
 Publication Status: Issued
 Pages: 204
 Publishing info: Jena : Friedrich-Schiller-Universität Jena, Philosophische Fakultät
 Table of Contents: 1. Introduction
2. Major faunal turnovers in Australia during the last 100,000 years
2.1. Late Pleistocene megafauna extinctions
2.1.1. Human arrival and extinction chronology
2.1.2. Causes of extinction - Competing hypotheses
2.2. Moving beyond megafauna – Extinctions during the Holocene
2.2.1. The introduction of the dingo
2.2.2. Human intensification
2.2.3. Climate change
2.3. Species introductions – Extinctions following European colonization
2.3.1. Introduction of non-native species
2.3.2. Pastoralism
2.3.3. Marginalization of indigenous land management practices
2.3.4. Direct human impacts
2.4. The potential of palaeoproteomics
3. Methods used for this thesis
3.1. Collagen structure and genetic diversity
3.2. Zooarchaeology by Mass Spectrometry (ZooMS)
3.2.1. Collagen extraction
3.2.2. MALDI-ToF-MS and taxonomic identification
3.3. Shotgun palaeoproteomics and de novo sequencing
3.3.1. De novo/error tolerant sequencing
3.4. Protein preservation
3.4.1. Thermal age estimates
3.4.2. Fourier transform infrared spectroscopy (FTIR)
3.4.3. Deamidation
4. Aims and objectives
5. Overview of manuscripts and author contributions
5.1. Manuscript A
5.2. Manuscript B
5.3. Manuscript C
 Rev. Type: -
 Identifiers: URN: https://www.db-thueringen.de/receive/dbt_mods_00057977
URI: http://uri.gbv.de/document/gvk:ppn:1852851171
Other: shh3417
 Degree: PhD

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