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  Author Correction: Effects of drought and recovery on soil volatile organic compound fluxes in an experimental rainforest

Pugliese, G., Ingrisch, J., Meredith, L. K., Pfannerstill, E. Y., Klüpfel, T., Meeran, K., et al. (2023). Author Correction: Effects of drought and recovery on soil volatile organic compound fluxes in an experimental rainforest. Nature Communications, 14: 6372. doi:10.1038/s41467-023-42207-4.

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 Creators:
Pugliese, Giovanni, Author
Ingrisch, Johannes, Author
Meredith, Laura K., Author
Pfannerstill, Eva Y.1, Author           
Klüpfel, Thomas1, Author           
Meeran, Kathiravan, Author
Byron, Joseph1, Author           
Purser, Gemma, Author
Gil-Loaiza, Juliana, Author
van Haren, Joost, Author
Dontsova, Katerina, Author
Kreuzwieser, Jürgen, Author
Ladd, S. Nemiah, Author
Werner, Christiane , Author
Williams, Jonathan1, Author           
Affiliations:
1Atmospheric Chemistry, Max Planck Institute for Chemistry, Max Planck Society, ou_1826285              

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 Abstract: The molecular architecture of alpha -Synuclein (alpha -Syn) inclusions, pathognomonic of various neurodegenerative disorders, remains unclear. alpha -Syn inclusions were long thought to consist mainly of alpha -Syn fibrils, but recent reports pointed to intracellular membranes as the major inclusion component. Here, we use cryo-electron tomography (cryo-ET) to image neuronal alpha -Syn inclusions in situ at molecular resolution. We show that inclusions seeded by alpha -Syn aggregates produced recombinantly or purified from patient brain consist of alpha -Syn fibrils crisscrossing a variety of cellular organelles. Using gold-labeled seeds, we find that aggregate seeding is predominantly mediated by small alpha -Syn fibrils, from which cytoplasmic fibrils grow unidirectionally. Detailed analysis of membrane interactions revealed that alpha -Syn fibrils do not contact membranes directly, and that alpha -Syn does not drive membrane clustering. Altogether, we conclusively demonstrate that neuronal alpha -Syn inclusions consist of alpha -Syn fibrils intermixed with membranous organelles, and illuminate the mechanism of aggregate seeding and cellular interaction. The molecular architecture of alpha -Synuclein (alpha -Syn) inclusions, pathognomonic of various neurodegenerative disorders, remains unclear. Here, authors use cryo-electron tomography to image neuronal alpha -Syn inclusions in situ and find that inclusions consist of alpha -Syn fibrils intermixed with cellular organelles without interacting directly.

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Language(s): eng - English
 Dates: 2023-10-11
 Publication Status: Published online
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1038/s41467-023-42207-4
 Degree: -

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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 14 Sequence Number: 6372 Start / End Page: - Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723