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  Identification of NAD-RNA species and ADPR-RNA decapping in Archaea

Gomes-Filho, J. V., Breuer, R., Morales-Filloy, H. G., Pozhydaieva, N., Borst, A., Paczia, N., et al. (2023). Identification of NAD-RNA species and ADPR-RNA decapping in Archaea. Nature Communications, 14: 7597. doi:10.1038/s41467-023-43377-x.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000D-FD72-6 Version Permalink: https://hdl.handle.net/21.11116/0000-000F-2F0B-2
Genre: Journal Article

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https://doi.org/10.1038/s41467-023-43377-x (Publisher version)
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 Creators:
Gomes-Filho, José Vicente1, Author
Breuer, Ruth1, Author
Morales-Filloy, Hector Gabriel1, Author
Pozhydaieva, Nadiia2, Author           
Borst, Andreas1, Author
Paczia, Nicole3, Author                 
Soppa, Jörg1, Author
Höfer, Katharina2, Author                 
Jäschke, Andres1, Author
Randau, Lennart1, Author
Affiliations:
1external, ou_persistent22              
2Max Planck Research Group Bacterial Epitranscriptomics, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266299              
3Core Facility Metabolomics and small Molecules Mass Spectrometry, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266267              

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 Abstract: NAD is a coenzyme central to metabolism that also serves as a 5′-terminal cap for bacterial and eukaryotic transcripts. Thermal degradation of NAD can generate nicotinamide and ADP-ribose (ADPR). Here, we use LC-MS/MS and NAD captureSeq to detect and identify NAD-RNAs in the thermophilic model archaeon Sulfolobus acidocaldarius and in the halophilic mesophile Haloferax volcanii. None of the four Nudix proteins of S. acidocaldarius catalyze NAD-RNA decapping in vitro, but one of the proteins (Saci_NudT5) promotes ADPR-RNA decapping. NAD-RNAs are converted into ADPR-RNAs, which we detect in S. acidocaldarius total RNA. Deletion of the gene encoding the 5′−3′ exonuclease Saci-aCPSF2 leads to a 4.5-fold increase in NAD-RNA levels. We propose that the incorporation of NAD into RNA acts as a degradation marker for Saci-aCPSF2. In contrast, ADPR-RNA is processed by Saci_NudT5 into 5′-p-RNAs, providing another layer of regulation for RNA turnover in archaeal cells.

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Language(s): eng - English
 Dates: 2022-11-082023-11-082023-11-21
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: URI: https://doi.org/10.1038/s41467-023-43377-x
Other: Gomes-Filho2023
DOI: 10.1038/s41467-023-43377-x
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Project name : -
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Funding program : Heisenberg program
Funding organization : Deutsche Forschungsgemeinschaft (DFG)
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Grant ID : -
Funding program : SPP 2330 (464500427)
Funding organization : Deutsche Forschungsgemeinschaft (DFG)
Project name : RTG 2355
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Funding program : -
Funding organization : Deutsche Forschungsgemeinschaft (DFG)
Project name : -
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Funding program : Diffusible Signals
Funding organization : LOEWE Research Cluster
Project name : -
Grant ID : 882789 RNA- Coenzyme
Funding program : Max Planck Research Group Leader funding
Funding organization : Max Planck Society
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Funding program : European Union’s Horizon 2020
Funding organization : European Research Council (ERC)
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Funding program : Open Access
Funding organization : DEAL

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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
 Creator(s):
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Publ. Info: London : Nature Publishing Group
Pages: 7597 Volume / Issue: 14 Sequence Number: 7597 Start / End Page: - Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723