Proteometabolomics of initial and recurrent glioblastoma highlights an 
increased immune cell signature with altered lipid metabolism

Cosenza-Contreras, Miguel; Schäfer, Agnes; Sing, Justin; Cook, Lena; Stillger, Maren N; Chen, Chia-Yi; Hidalgo, Jose Villacorta; Pinter, Niko; Meyer, Larissa; Werner, Tilman; Bug, Darleen; Haberl, Zeno; Kübeck, Oliver; Zhao, Kai; Stei, Susanne; Gafencu, Anca Violeta; Ionita, Radu; Brehar, Felix M; Ferrer-Lozano, Jaime; Ribas, Gloria; Cerdá-Alberich, Leo; Martí-Bonmatí, Luis; Nimsky, Christopher; Straaten, Alexis Van; Biniossek, Martin L; Föll, Melanie; Cabezas-Wallscheid, Nina; Büscher, Jörg Martin; Röst, Hannes; Arnoux, Armelle; Bartsch, Jörg W; Schilling, Oliver

doi:10.1093/neuonc/noad208

Local TagsRelease HistoryDetailsSummary

Proteometabolomics of initial and recurrent glioblastoma highlights an increased immune cell signature with altered lipid metabolism

Cosenza-Contreras, M., Schäfer, A., Sing, J., Cook, L., Stillger, M. N., Chen, C.-Y., et al. (2023). Proteometabolomics of initial and recurrent glioblastoma highlights an increased immune cell signature with altered lipid metabolism. Neuro-Oncology: official journal of the World Federation of Neuro-Oncology, noad208. doi:10.1093/neuonc/noad208.

Item is Released

show all hide all

Basic

show hide

Item Permalink: https://hdl.handle.net/21.11116/0000-000D-FEF4-2 Version Permalink: https://hdl.handle.net/21.11116/0000-000D-FEF5-1

Genre: Journal Article

Files

show Files

hide Files

:

10.1093_neuonc_noad208.pdf (Publisher version), 3MB

File Permalink:
-

Name:
10.1093_neuonc_noad208.pdf

Description:
-

OA-Status:

Visibility:
Restricted (Max Planck Institute of Immunobiology and Epigenetics, MFIB; )

MIME-Type / Checksum:
application/pdf

Technical Metadata:

Copyright Date:
2023

Copyright Info:
The Author(s) 2023. Published by Oxford University Press on behalf of the Society for Neuro-Oncology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com

License:
-

Locators

show

hide

Locator:
https://academic.oup.com/neuro-oncology/advance-article/doi/10.1093/neuonc/noad208/7330381?login=true (Publisher version) Open Access status unknown

Description:
-

OA-Status:
Not specified

Creators

show

hide

Creators:
Cosenza-Contreras, Miguel¹, Author
Schäfer, Agnes¹, Author
Sing, Justin¹, Author
Cook, Lena¹, Author
Stillger, Maren N¹, Author
Chen, Chia-Yi¹, Author
Hidalgo, Jose Villacorta¹, Author
Pinter, Niko¹, Author
Meyer, Larissa¹, Author
Werner, Tilman¹, Author
Bug, Darleen¹, Author
Haberl, Zeno¹, Author
Kübeck, Oliver¹, Author
Zhao, Kai¹, Author
Stei, Susanne¹, Author
Gafencu, Anca Violeta¹, Author
Ionita, Radu¹, Author
Brehar, Felix M¹, Author
Ferrer-Lozano, Jaime¹, Author
Ribas, Gloria¹, Author
Cerdá-Alberich, Leo¹, AuthorMartí-Bonmatí, Luis¹, AuthorNimsky, Christopher¹, AuthorStraaten, Alexis Van¹, AuthorBiniossek, Martin L¹, AuthorFöll, Melanie¹, AuthorCabezas-Wallscheid, Nina², Author Büscher, Jörg Martin³, Author Röst, Hannes¹, AuthorArnoux, Armelle¹, AuthorBartsch, Jörg W¹, AuthorSchilling, Oliver¹, Author more..

Affiliations:
1External Organizations, ou_persistent22
2Department of Cellular and Molecular Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243641
3Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243641

Content

show

hide

Free keywords: acid ceramidase (ASAH1); glioblastoma; lipidomics; proteomics; tumor microenvironment.

Abstract: Background: There is an urgent need to better understand the mechanisms associated with the development, progression, and onset of recurrence after initial surgery in glioblastoma (GBM). The use of integrative phenotype-focused -omics technologies such as proteomics and lipidomics provides an unbiased approach to explore the molecular evolution of the tumor and its associated environment.

Methods: We assembled a cohort of patient-matched initial (iGBM) and recurrent (rGBM) specimens of resected GBM. Proteome and metabolome composition were determined by mass spectrometry-based techniques. We performed neutrophil-GBM cell co-culture experiments to evaluate the behavior of rGBM-enriched proteins in the tumor microenvironment. ELISA-based quantitation of candidate proteins was performed to test the association of their plasma concentrations in iGBM with the onset of recurrence.

Results: Proteomic profiles reflect increased immune cell infiltration and extracellular matrix reorganization in rGBM. ASAH1, SYMN, and GPNMB were highly enriched proteins in rGBM. Lipidomics indicates the downregulation of ceramides in rGBM. Cell analyses suggest a role for ASAH1 in neutrophils and its localization in extracellular traps. Plasma concentrations of ASAH1 and SYNM show an association with time-to-recurrence.

Conclusions: We describe the potential importance of ASAH1 in tumor progression and development of rGBM via metabolic rearrangement and showcase the feedback from the tumor microenvironment to plasma proteome profiles. We report the potential of ASAH1 and SYNM as plasma markers of rGBM progression. The published datasets can be considered as a resource for further functional and biomarker studies involving additional -omics technologies.

Details

show

hide

Language(s): eng - English

Dates: Published Online: 2023-10-26

Publication Status: Published online

Pages: -

Publishing info: -

Table of Contents: -

Rev. Type: -

Identifiers: DOI: 10.1093/neuonc/noad208

Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show

hide

Title: Neuro-Oncology : official journal of the World Federation of Neuro-Oncology

Other : Neuro-Oncology

Abbreviation : Neuro Oncol

Source Genre: Journal

Creator(s):

Affiliations:

Publ. Info: USA : Oxford : Oxford Univ. Press

Pages: - Volume / Issue: - Sequence Number: noad208 Start / End Page: - Identifier: ISSN: 1523-5866
CoNE: https://pure.mpg.de/cone/journals/resource/1523-5866