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  mRNA Transfection of S. mediterranea for Luminescence Analysis

Weill, U., Hall, R. N., Drees, L., Wang, B., & Rink, J. C. (2023). mRNA Transfection of S. mediterranea for Luminescence Analysis. In L. Gentile (Ed.), Schmidtea Mediterranea: Methods and Protocols (pp. 263-275). New York, NY: Springer US. doi:10.1007/978-1-0716-3275-8_17.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000E-06CF-3 Version Permalink: https://hdl.handle.net/21.11116/0000-000E-06D0-0
Genre: Book Chapter

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978-1-0716-3275-8_17.pdf (Publisher version), 393KB
 
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 Creators:
Weill, Uri1, Author           
Hall, Richard Nelson, Author
Drees, Leonard1, Author           
Wang, Bo, Author
Rink, Jochen C.1, Author           
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1Department of Tissue Dynamics and Regeneration, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society, ou_3350274              

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 Abstract: Planarians have become a powerful model system for stem cell research and regeneration. While the tool kit for mechanistic investigations has been steadily expanding over the last decade, robust genetic tools for transgene expression are still lacking. We describe here methods for in vivo and in vitro mRNA transfection of the planarian species Schmidtea mediterranea. These methods utilize the commercially available TransIT-mRNA transfection reagent to efficiently deliver mRNA encoding a synthetic nanoluciferase reporter. Using a luminescent reporter overcomes the bright autofluorescent background of planarian tissues and allows quantitative measurements of protein expression levels. Collectively, our methods provide the means for heterologous reporter expression in planarian cells and the basis for future development of transgenic techniques.

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Language(s): eng - English
 Dates: 2023-07-112023
 Publication Status: Issued
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1007/978-1-0716-3275-8_17
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Title: Schmidtea Mediterranea: Methods and Protocols
Source Genre: Book
 Creator(s):
Gentile, Luca, Editor
Affiliations:
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Publ. Info: New York, NY : Springer US
Pages: - Volume / Issue: 2680 Sequence Number: - Start / End Page: 263 - 275 Identifier: ISSN: 1064-3745
ISSN: 1940-6029
ISBN: 978-1-0716-3274-1
ISBN: 978-1-0716-3275-8

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Title: Methods in Molecular Biology
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Pages: - Volume / Issue: 2680 Sequence Number: - Start / End Page: - Identifier: -