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  Whole-Mount In Situ Hybridization in Large Sexual Schmidtea mediterranea

Vila-Farré, M., Vu, H.-T.-K., & Rink, J. C. (2023). Whole-Mount In Situ Hybridization in Large Sexual Schmidtea mediterranea. In L. Gentile (Ed.), Schmidtea Mediterranea: Methods and Protocols (pp. 107-119). New York, NY: Springer US. doi:10.1007/978-1-0716-3275-8_7.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000E-06DF-1 Version Permalink: https://hdl.handle.net/21.11116/0000-000E-06E0-E
Genre: Book Chapter

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978-1-0716-3275-8_7.pdf (Publisher version), 290KB
 
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 Creators:
Vila-Farré, Miquel1, Author           
Vu, Hanh Thi-Kim, Author
Rink, Jochen C.1, Author           
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1Department of Tissue Dynamics and Regeneration, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society, ou_3350274              

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 Abstract: Whole-mount in situ hybridization (WISH), colorimetric or fluorescent (FISH), allows for the visualization of endogenous RNA. For planarians, robust WISH protocols exist for small-sized animals (>5 mm) of the model species Schmidtea mediterranea and Dugesia japonica. However, the sexual strain of Schmidtea mediterranea studied for germline development and function reaches much larger body sizes in excess of 2 cm. The existing whole-mount WISH protocols are not optimal for such large specimens, owing to insufficient tissue permeabilization. Here, we describe a robust WISH protocol for 12–16 mm long sexually mature Schmidtea mediterranea individuals that could serve as a starting point for adapting WISH to other large planarian species.

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Language(s): eng - English
 Dates: 2023-07-112023
 Publication Status: Issued
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1007/978-1-0716-3275-8_7
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Title: Schmidtea Mediterranea: Methods and Protocols
Source Genre: Book
 Creator(s):
Gentile, Luca, Editor
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Publ. Info: New York, NY : Springer US
Pages: - Volume / Issue: 2680 Sequence Number: - Start / End Page: 107 - 119 Identifier: ISSN: 1064-3745
ISSN: 1940-6029
ISBN: 978-1-0716-3274-1
ISBN: 978-1-0716-3275-8

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Title: Methods in Molecular Biology
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Pages: - Volume / Issue: 2680 Sequence Number: - Start / End Page: - Identifier: -