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  Cytosolic sorting platform complexes shuttle type III secretion system effectors to the injectisome in Yersinia enterocolitica

Wimmi, S., Balinovic, A., Brianceau, C. F., Pintor, K. L., Vielhauer, J., Turkowyd, B., Helbig, C., Fleck, M., Langenfeld, K., Kahnt, J., Glatter, T., Endesfelder, U., & Diepold, A. (2024). Cytosolic sorting platform complexes shuttle type III secretion system effectors to the injectisome in Yersinia enterocolitica. Nature Microbiology, 9, 185-199. doi:10.1038/s41564-023-01545-1.

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アイテムのパーマリンク: https://hdl.handle.net/21.11116/0000-000E-1AA0-0 版のパーマリンク: https://hdl.handle.net/21.11116/0000-000F-2F08-5
資料種別: 学術論文

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 作成者:
Wimmi, Stephan1, 著者           
Balinovic, Alexander2, 著者           
Brianceau, Corentin Florian1, 著者           
Pintor, Katherine Lapis1, 著者           
Vielhauer, Jan1, 著者           
Turkowyd, Bartosz3, 著者           
Helbig, Carlos1, 著者           
Fleck, Moritz1, 著者           
Langenfeld, Katja1, 著者           
Kahnt, Jörg4, 著者           
Glatter, Timo4, 著者                 
Endesfelder, Ulrike3, 5, 著者           
Diepold, Andreas1, 著者                 
所属:
1Research Group Bacterial Secretion Systems, Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266306              
2Microbial Networks, Department of Systems and Synthetic Microbiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266309              
3Department of Systems and Synthetic Microbiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266288              
4Core Facility Mass Spectrometry and Proteomics, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266266              
5external, ou_persistent22              

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 要旨: Bacteria use type III secretion injectisomes to inject effector proteins into eukaryotic target cells. Recruitment of effectors to the machinery and the resulting export hierarchy involve the sorting platform. These conserved proteins form pod structures at the cytosolic interface of the injectisome but are also mobile in the cytosol. Photoactivated localization microscopy in Yersinia enterocolitica revealed a direct interaction of the sorting platform proteins SctQ and SctL with effectors in the cytosol of live bacteria. These proteins form larger cytosolic protein complexes involving the ATPase SctN and the membrane connector SctK. The mobility and composition of these mobile pod structures are modulated in the presence of effectors and their chaperones, and upon initiation of secretion, which also increases the number of injectisomes from ~5 to ~18 per bacterium. Our quantitative data support an effector shuttling mechanism, in which sorting platform proteins bind to effectors in the cytosol and deliver the cargo to the export gate at the membrane-bound injectisome.

資料詳細

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言語: eng - English
 日付: 2023-11-062024
 出版の状態: 出版
 ページ: -
 出版情報: -
 目次: -
 査読: 査読あり
 識別子(DOI, ISBNなど): URI: https://doi.org/10.1038/s41564-023-01545-1
その他: Wimmi2024
DOI: 10.1038/s41564-023-01545-1
 学位: -

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Project information

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Project name : -
Grant ID : -
Funding program : -
Funding organization : Max Planck Society
Project name : -
Grant ID : DI 1765/5-1
Funding program : Foundation CELLEX
Funding organization : Max Planck Society
Project name : -
Grant ID : PPP USA 2021-2023
Funding program : -
Funding organization : German Research Foundation (DFG)
Project name : -
Grant ID : -
Funding program : -
Funding organization : Deutscher Akademischer Austausch Dienst (DAAD)
Project name : -
Grant ID : -
Funding program : -
Funding organization : Max Planck Institute for Terrestrial Microbiology, Marburg

出版物 1

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出版物名: Nature Microbiology
  省略形 : Nat. Microbiol.
種別: 学術雑誌
 著者・編者:
所属:
出版社, 出版地: London, UK : Nature Publishing Group
ページ: - 巻号: 9 通巻号: - 開始・終了ページ: 185 - 199 識別子(ISBN, ISSN, DOIなど): ISSN: 2058-5276
CoNE: https://pure.mpg.de/cone/journals/resource/2058-5276