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要旨:
Neuropeptides are neuronal signaling molecules present in almost all animal taxa. They are important modulators of many different behaviors and physiological processes, including sensorimotor integration, central pattern generation, circadian rhythms, feeding and metabolism, and reproductive behaviors. Neuropeptidergic signaling is very widespread in the nervous system, and most animals possess numerous proneuro- peptide genes. To better understand the complexity of neuropeptide signaling in the context of an entire organism, we are making a full body cellular resolution gene expression atlas for 98 neuropeptides identi- fied in the marine annelid Platyneris dumerilii. We obtained expression patterns by whole mount in situ hybridization of two distinct develop290 mental stages. This enables us to visualize differences in neuropeptide signaling during a major developmental transition in the life of a marine annelid. In addition to neuropeptides, we also generated expression pat- terns for marker genes for some of the classical neurotransmitters (e.g. acetylcholine, glutamate) for coexpression analyses. Furthermore, we plan to combine the neuropeptide expression atlas with a full body serial sectioned electron microscopy dataset generated in our lab to recon- struct neuropeptidergic neurons and their neural circuitry. Finally, under- standing neuropeptidergic connectivity, combined with the knowledge of the evolutionary histories of neuropeptides and organs, can provide valuable insight into the evolution of behavior.
