English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Direct observation of a condensate effect on super-enhancer controlled gene bursting

Du, M., Stitzinger, S. H., Spille, J.-H., Cho, W.-K., Lee, C., Hijaz, M., et al. (2024). Direct observation of a condensate effect on super-enhancer controlled gene bursting. Cell, 187, 331-344. doi:10.1016/j.cell.2023.12.005.

Item is

Files

show Files
hide Files
:
10.1016_j.cell.2023.12.005.pdf (Publisher version), 8MB
Name:
10.1016_j.cell.2023.12.005.pdf
Description:
-
OA-Status:
Not specified
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
2023
Copyright Info:
The Authors. Published by Elsevier Inc. All rights reserved.

Locators

show
hide
Description:
-
OA-Status:
Not specified

Creators

show
hide
 Creators:
Du, Manyu1, Author           
Stitzinger, Simon Hendrik1, Author
Spille, Jan-Hendrik1, Author
Cho, Won-Ki1, Author
Lee, Choongman1, Author
Hijaz, Mohammed1, Author
Quintana, Andrea1, Author
Cissé, Ibrahim Ibrahim1, Author           
Affiliations:
1Department of Biological Physics, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_persistent22              

Content

show
hide
Free keywords: RNA polymerase II; Sox2; biomolecular condensate; cluster; cohesin; function; nuclear organization; phase separation; super-enhancer; transcription.
 Abstract: Enhancers are distal DNA elements believed to loop and contact promoters to control gene expression. Recently, we found diffraction-sized transcriptional condensates at genes controlled by clusters of enhancers (super-enhancers). However, a direct function of endogenous condensates in controlling gene expression remains elusive. Here, we develop live-cell super-resolution and multi-color 3D-imaging approaches to investigate putative roles of endogenous condensates in the regulation of super-enhancer controlled gene Sox2. In contrast to enhancer distance, we find instead that the condensate's positional dynamics are a better predictor of gene expression. A basal gene bursting occurs when the condensate is far (>1 μm), but burst size and frequency are enhanced when the condensate moves in proximity (<1 μm). Perturbations of cohesin and local DNA elements do not prevent basal bursting but affect the condensate and its burst enhancement. We propose a three-way kissing model whereby the condensate interacts transiently with gene locus and regulatory DNA elements to control gene bursting.

Details

show
hide
Language(s): eng - English
 Dates: 2024-01-08
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1016/j.cell.2023.12.005
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Cell
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 187 Sequence Number: - Start / End Page: 331 - 344 Identifier: ISSN: 0092-8674
CoNE: https://pure.mpg.de/cone/journals/resource/954925463183