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  Regularly spaced tyrosines in EBF1 mediate BRG1 recruitment and formation of nuclear subdiffractive clusters

Zolotarev, N., Wang, Y., Du, M., Bayer, M., Grosschedl, A., Cissé, I. I., et al. (2024). Regularly spaced tyrosines in EBF1 mediate BRG1 recruitment and formation of nuclear subdiffractive clusters. Genes and Development. doi:10.1101/gad.350828.123.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000E-4140-0 Version Permalink: https://hdl.handle.net/21.11116/0000-000E-4141-F
Genre: Journal Article

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10.1101_gad.350828.123.pdf (Publisher version), 4MB
 
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2024
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Zolotarev et al.; Published by Cold Spring Harbor Laboratory Press. This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License
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https://genesdev.cshlp.org/content/early/2024/01/17/gad.350828.123 (Publisher version)
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 Creators:
Zolotarev, Nikolay1, Author
Wang, Yuanting1, Author
Du, Manyu2, Author           
Bayer, Marc1, Author
Grosschedl, Anna1, Author
Cissé, Ibrahim Ibrahim2, Author           
Grosschedl, Rudolf1, Author           
Affiliations:
1Department of Cellular and Molecular Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243641              
2Department of Biological Physics, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_persistent22              

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Free keywords: B cell differentiation; BRG1; EBF1; IDR; chromatin; condensates; phase separation
 Abstract: B lineage priming by pioneer transcription factor EBF1 requires the function of an intrinsically disordered region (IDR). Here, we examine the role of regularly spaced tyrosines in the IDR as potential determinants of IDR function and activity of EBF1. We found that four Y > A mutations in EBF1 reduced the formation of condensates in vitro and subdiffractive clusters in vivo. Notably, Y > A mutant EBF1 was inefficient in promoting B cell differentiation and showed impaired chromatin binding, recruitment of BRG1, and activation of specific target genes. Thus, regularly spaced tyrosines in the IDR contribute to the biophysical and functional properties of EBF1.

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Language(s): eng - English
 Dates: 2024-01-17
 Publication Status: Published online
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1101/gad.350828.123
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Title: Genes and Development
Source Genre: Journal
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Publ. Info: Cold Spring Harbor Laboratory Press
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: ISSN: 0890-9369
CoNE: https://pure.mpg.de/cone/journals/resource/954925557453