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  Two paralogous PHD finger proteins participate in Paramecium tetraurelia’s natural genome editing

Häußermann, L., Singh, A., & Swart, E. (submitted). Two paralogous PHD finger proteins participate in Paramecium tetraurelia’s natural genome editing.

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Häußermann, L1, Autor           
Singh, A1, Autor                 
Swart, EC1, Autor                 
Affiliations:
1Research Group Ciliate Genomics and Molecular Biology, Max Planck Institute for Biology Tübingen, Max Planck Society, ou_3375053              

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 Zusammenfassung: The unicellular eukaryote Paramecium tetraurelia contains functionally distinct nuclei: germline micronuclei (MICs) and a somatic macronucleus (MAC). During sexual reproduction, the MIC genome is reorganized into a new MAC genome and the old MAC is lost. Almost 45,000 unique Internal Eliminated Sequences (IESs) distributed throughout the genome require precise excision to guarantee a functional new MAC genome. Here, we characterize a pair of paralogous PHD finger proteins involved in DNA elimination. DevPF1, the early-expressed paralog, is present in only some of the gametic and post-zygotic nuclei during meiosis. Both DevPF1 and DevPF2 localize in the new developing MACs, where IESs excision occurs. In DevPF2 knockdown (KD) long IESs are preferentially retained and late-expressed small RNAs decrease; no length preference for retained IESs was observed in DevPF1-KD and development-specific small RNAs were abolished. The expression of at least two genes from the new MAC with roles in genome reorganization seems to be influenced by DevPF1- and DevPF2-KD. Thus, both PHD fingers are crucial for new MAC genome development, with distinct functions, potentially via regulation of non-coding and coding transcription in the MICs and new MACs.

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 Datum: 2024-01
 Publikationsstatus: Eingereicht
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 Identifikatoren: DOI: 10.1101/2024.01.23.576875
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