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  Analytical improvements and assessment of long-term performance of the oxidation-denitrifier method

Moretti, S., Duprey, N. N., Foreman, A. D., Arns, A., Brömme, S., Jung, J., Ai, X. E., Auderset, A., Bieler, A. L., Eck, C., Farmer, J., Hinnenberg, B., Lacerra, M., Leichliter, J., Lüdecke, T., Oleynik, S., Rubach, F., Schmitt, M., Vink, M., Wald, T., Yehudai, M., Sigman, D. M., & Martinez-Garcia, A. (2024). Analytical improvements and assessment of long-term performance of the oxidation-denitrifier method. Rapid Communications in Mass Spectrometry, 38(1):. doi:10.1002/rcm.9650.

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アイテムのパーマリンク: https://hdl.handle.net/21.11116/0000-000E-51B8-7 版のパーマリンク: https://hdl.handle.net/21.11116/0000-000E-51B9-6
資料種別: 学術論文

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作成者

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 作成者:
Moretti, Simone1, 著者           
Duprey, Nicolas N.1, 著者           
Foreman, Alan D.1, 著者           
Arns, Anthea1, 著者           
Brömme, Sven1, 著者           
Jung, Jonathan1, 著者           
Ai, Xuyuan E.1, 著者           
Auderset, Alexandra1, 著者           
Bieler, Aaron L.1, 著者           
Eck, Camino1, 著者           
Farmer, Jesse1, 著者           
Hinnenberg, Barbara1, 著者           
Lacerra, Matthew1, 著者           
Leichliter, Jennifer1, 著者           
Lüdecke, Tina1, 著者           
Oleynik , Sergey2, 著者
Rubach, Florian3, 著者           
Schmitt, Mareike1, 著者           
Vink, Marissa2, 著者
Wald, Tanja1, 著者           
Yehudai, Maayan1, 著者           Sigman , Daniel M.2, 著者Martinez-Garcia, Alfredo1, 著者            全て表示
所属:
1Climate Geochemistry, Max Planck Institute for Chemistry, Max Planck Society, ou_2237635              
2external, ou_persistent22              
3Particle Chemistry, Max Planck Institute for Chemistry, Max Planck Society, ou_1826291              

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 要旨:

The analysis of the nitrogen (N) isotopic composition of organic matter bound to fossil biomineral structures (BB-δ15N) using the oxidation–denitrifier (O–D) method provides a novel tool to study past changes in N cycling processes.
Methods

We report a set of methodological improvements to the O–D method, including (a) a method for sealing the reaction vials in which the oxidation of organic N to NO3− takes place, (b) a recipe for bypassing the pH adjustment step before the bacterial conversion of NO3− to N2O, and (c) a method for storing recrystallized dipotassium peroxodisulfate (K2S2O8) under Ar atmosphere.
Results

The new sealing method eliminates the occasional contamination and vial breakage that occurred previously while increasing sample throughput. The protocol for bypassing pH adjustment does not affect BB-δ15N, and it significantly reduces the processing time. Storage of K2S2O8 reagent under Ar atmosphere produces stable oxidation blanks over more than 3.5 years. We report analytical blanks, accuracy, and precision for this methodology from eight users over the course of ~3.5 years of analyses at the Max Planck Institute for Chemistry. Our method produces analytical blanks characterized by low N content (0.30 ± 0.13 nmol N, 1σ, n = 195) and stable δ15N (−2.20 ± 3.13‰, n = 195). The analysis of reference amino acid standards USGS 40 and USGS 65 indicates an overall accuracy of −0.23 ± 0.35‰ (1σ, n = 891). The analysis of in-house fossil standards gives similar analytical precision (1σ) across a range of BB-δ15N values and biominerals: zooxanthellate coral standard PO-1 (6.08 ± 0.21‰, n = 267), azooxanthellate coral standard LO-1 (10.20 ± 0.28‰, n = 258), foraminifera standard MF-1 (5.92 ± 0.28‰, n = 243), and tooth enamel AG-Lox (4.06 ± 0.49‰, n = 78).
Conclusions

The methodological improvements significantly increase sample throughput without compromising analytical precision or accuracy down to 1 nmol of N.

資料詳細

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言語: eng - English
 日付: 2023-12-062024
 出版の状態: 出版
 ページ: -
 出版情報: -
 目次: -
 査読: -
 識別子(DOI, ISBNなど): ISI: 001121271200001
DOI: 10.1002/rcm.9650
 学位: -

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出版物 1

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出版物名: Rapid Communications in Mass Spectrometry
  その他 : Rapid Commun. Mass Spectrom.
種別: 学術雑誌
 著者・編者:
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出版社, 出版地: New York, NY : John Wiley & Sons
ページ: 19 巻号: 38 (1) 通巻号: e9650 開始・終了ページ: - 識別子(ISBN, ISSN, DOIなど): ISSN: 0951-4198
CoNE: https://pure.mpg.de/cone/journals/resource/954925574961