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  Landscape of protein-protein interactions during hepatitis C virus assembly and release

Matthaei, A., Joecks, S., Frauenstein, A., Bruening, J., Bankwitz, D., Friesland, M., et al. (2024). Landscape of protein-protein interactions during hepatitis C virus assembly and release. Microbiology Spectrum. doi:10.1128/spectrum.02562-22.

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 Creators:
Matthaei, Alina, Author
Joecks, Sebastian, Author
Frauenstein, Annika1, Author           
Bruening, Janina, Author
Bankwitz, Dorothea, Author
Friesland, Martina, Author
Gerold, Gisa, Author
Vieyres, Gabrielle, Author
Kaderali, Lars, Author
Meissner, Felix1, Author           
Pietschmann, Thomas, Author
Das, Saumitra, Author
Affiliations:
1Meissner, Felix / Experimental Systems Immunology, Max Planck Institute of Biochemistry, Max Planck Society, ou_2149678              

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Free keywords: RETICULUM-ASSOCIATED DEGRADATION; ENDOPLASMIC-RETICULUM; CORE PROTEIN; P7 PROTEIN; SUBCELLULAR-LOCALIZATION; STRUCTURAL DETERMINANTS; FUNCTIONAL ASSOCIATIONS; TRANSMEMBRANE DOMAIN; INTERACTION NETWORKS; PARTICLE FORMATIONMicrobiology; hepatitis C virus; viral assembly and release; proteomics; affinity purification; endoplasmic reticulum; ERAD; HSPA5; Rad23B; host-pathogen interactions; virus-host interactions; lipoproteins;
 Abstract: Assembly of infectious hepatitis C virus (HCV) particles requires multiple cellular proteins including for instance apolipoprotein E (ApoE). To describe these protein-protein interactions, we performed an affinity purification mass spectrometry screen of HCV-infected cells. We used functional viral constructs with epitope-tagged envelope protein 2 (E2), protein (p) 7, or nonstructural protein 4B (NS4B) as well as cells expressing a tagged variant of ApoE. We also evaluated assembly stage-dependent remodeling of protein complexes by using viral mutants carrying point mutations abrogating particle production at distinct steps of the HCV particle production cascade. Five ApoE binding proteins, 12 p7 binders, 7 primary E2 interactors, and 24 proteins interacting with NS4B were detected. Cell-derived PREB, STT3B, and SPCS2 as well as viral NS2 interacted with both p7 and E2. Only GTF3C3 interacted with E2 and NS4B, highlighting that HCV assembly and replication complexes exhibit largely distinct interactomes. An HCV core protein mutation, preventing core protein decoration of lipid droplets, profoundly altered the E2 interactome. In cells replicating this mutant, E2 interactions with HSPA5, STT3A/B, RAD23A/B, and ZNF860 were significantly enhanced, suggesting that E2 protein interactions partly depend on core protein functions. Bioinformatic and functional studies including STRING network analyses, RNA interference, and ectopic expression support a role of Rad23A and Rad23B in facilitating HCV infectious virus production. Both Rad23A and Rad23B are involved in the endoplasmic reticulum (ER)-associated protein degradation (ERAD). Collectively, our results provide a map of host proteins interacting with HCV assembly proteins, and they give evidence for the involvement of ER protein folding machineries and the ERAD pathway in the late stages of the HCV replication cycle.IMPORTANCEHepatitis C virus (HCV) establishes chronic infections in the majority of exposed individuals. This capacity likely depends on viral immune evasion strategies. One feature likely contributing to persistence is the formation of so-called lipo-viro particles. These peculiar virions consist of viral structural proteins and cellular lipids and lipoproteins, the latter of which aid in viral attachment and cell entry and likely antibody escape. To learn about how lipo-viro particles are coined, here, we provide a comprehensive overview of protein-protein interactions in virus-producing cells. We identify numerous novel and specific HCV E2, p7, and cellular apolipoprotein E-interacting proteins. Pathway analyses of these interactors show that proteins participating in processes such as endoplasmic reticulum (ER) protein folding, ER-associated protein degradation, and glycosylation are heavily engaged in virus production. Moreover, we find that the proteome of HCV replication sites is distinct from the assembly proteome, suggesting that transport process likely shuttles viral RNA to assembly sites.
Hepatitis C virus (HCV) establishes chronic infections in the majority of exposed individuals. This capacity likely depends on viral immune evasion strategies. One feature likely contributing to persistence is the formation of so-called lipo-viro particles. These peculiar virions consist of viral structural proteins and cellular lipids and lipoproteins, the latter of which aid in viral attachment and cell entry and likely antibody escape. To learn about how lipo-viro particles are coined, here, we provide a comprehensive overview of protein-protein interactions in virus-producing cells. We identify numerous novel and specific HCV E2, p7, and cellular apolipoprotein E-interacting proteins. Pathway analyses of these interactors show that proteins participating in processes such as endoplasmic reticulum (ER) protein folding, ER-associated protein degradation, and glycosylation are heavily engaged in virus production. Moreover, we find that the proteome of HCV replication sites is distinct from the assembly proteome, suggesting that transport process likely shuttles viral RNA to assembly sites.

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Language(s): eng - English
 Dates: 2024
 Publication Status: Published online
 Pages: 25
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 001143555400001
DOI: 10.1128/spectrum.02562-22
 Degree: -

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Title: Microbiology Spectrum
  Abbreviation : Microbiol. Spectr.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: American Society for Microbiology
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: ISSN: 2165-0497
CoNE: https://pure.mpg.de/cone/journals/resource/2165-0497