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要旨:
Tracking nanoparticle movement is highly desirable in many scientific areas, and various imaging
methods have been employed to achieve this goal. Interferometric scattering (iSCAT) microscopy has
been particularly successful in combining very high spatial and temporal resolution for tracking small
nanoparticles in all three dimensions. However, previous works have been limited to an axial range
of only a few hundred nanometers. Here, we present a robust and efficient strategy for localizing
nanoparticles recorded in high-speed iSCAT videos in three dimensions over tens of micrometers. We
showcase the performance of our algorithm by tracking gold nanoparticles as small as 10 nm diffusing
in water while maintaining 5 μs temporal resolution and nanometer axial localization precision. Our
results hold promise for applications in cell biology and material science, where the three-dimensional
motion of nanoparticles in complex media is of interest
